Harnessing next-generation molecular technologies for precision control of Tuta absoluta and Helicoverpa armigera in tomato

IF 4 4区生物学 Q1 GENETICS & HEREDITY

Functional & Integrative Genomics Pub Date : 2026-09-29 DOI:10.1007/s10142-026-02064-y

Amina Amin Ch., Noman Nazik, Muniba Shahid, Misbah Saleem, Areesha Nadeem, Faisal Saeed, Abdul Qayyum Rao, Iqbal Rashid, Allah Bakhsh

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引用次数: 0

Abstract

Tomato (Solanum lycopersicum) is one of the most significant vegetable crops cultivated around the world. Among biotic stresses, production of tomato is severely affected by insect pests mainly by tomato fruit borer (Helicoverpa armigera) and tomato leaf miner (Tuta absoluta). Traditionally to manage the destruction caused by insect pests, farmers mostly used chemical insecticides and by different cultural practices. Use of chemical pesticides is of great concern as it is harmful for the environment and other living beings and with the passage of time, insects develop resistance against pesticides. Latest progress in molecular biology enables scientists to develop next generation pest management approaches that are more precise, environment friendly and sustainable. This review describes nascent molecular approaches to manage tomato pests, like transgenic expression of insecticidal proteins such as of Bacillus thuringiensis (Bt) toxins, RNAi (RNA interference) strategies including HIGS and SIGS, genome editing by CRISPR-Cas, protease inhibitors and male sterility. These strategies not only enhance resistance of crop against pests but also are safe for environment and other organisms. This review also addresses the combination of different molecular approaches and pyramiding of genes involve in delaying resistance. Overall, these advanced molecular approaches providing sustainable resistance against insect pests have been discussed.

Graphical Abstract

查看原文本刊更多论文

利用新一代分子技术精确控制番茄中的绝对灰蛾和棉铃虫。

番茄（Solanum lycopersicum）是世界上最重要的蔬菜作物之一。在生物胁迫中，影响番茄生产的主要害虫是番茄果螟（Helicoverpa armigera）和番茄叶螨（Tuta absoluta）。传统上，为了控制虫害造成的破坏，农民大多使用化学杀虫剂和不同的文化习俗。化学农药的使用引起了人们的极大关注，因为它对环境和其他生物有害，而且随着时间的推移，昆虫会对农药产生抗药性。分子生物学的最新进展使科学家们能够开发出更精确、更环保、更可持续的下一代有害生物管理方法。本文综述了番茄害虫管理的新兴分子方法，如苏云金芽孢杆菌（Bacillus thuringiensis, Bt）毒素等杀虫蛋白的转基因表达、包括HIGS和SIGS在内的RNAi （RNA干扰）策略、CRISPR-Cas基因组编辑、蛋白酶抑制剂和雄性不育。这些策略不仅提高了作物对害虫的抵抗力，而且对环境和其他生物安全。本文还讨论了不同分子途径的结合和基因的金字塔化参与延迟抗性。总之，这些先进的分子方法提供了可持续的抗虫害的讨论。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Functional & Integrative Genomics 生物-遗传学

CiteScore

3.50

自引率

3.40%

发文量

审稿时长

2 months

期刊介绍： Functional & Integrative Genomics is devoted to large-scale studies of genomes and their functions, including systems analyses of biological processes. The journal will provide the research community an integrated platform where researchers can share, review and discuss their findings on important biological questions that will ultimately enable us to answer the fundamental question: How do genomes work?