Waseem Ahmed, Rafia Azmat, Abdul Qayyum, Hadi Abbas, Mohamed Fawzy Ramadan
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引用次数: 0
Abstract
Natural plant-derived compounds are increasingly explored as alternatives for managing skin disorders associated with oxidative stress, enzymatic degradation, and fibroblast dysfunction. In this study, bioactive constituents from Adhatoda vasica and Calotropis procera were isolated and characterized using UF-HPLC-DAD and Fourier-transform infrared (FTIR) spectroscopy. Identified compounds included viscic acid, pasakbumin C, 8′Z-enyl congeners, 5-(8′Z,11′Z-heptadecadienyl)-1,3-benzenediol, 9′-(o-methyl) protocetraric acid, and calophynic acid. The biological activities of leaf and seed extracts were evaluated through in vitro enzymatic assays targeting tyrosinase (monophenolase and diphenolase), collagenase, elastase, and hyaluronidase. A. vasica leaf extracts exhibited notable tyrosinase inhibition, with IC₅₀ values of 22.16 µg/mL for monophenolase and 15.5 µg/mL for diphenolase, while C. procera also demonstrated significant inhibitory activity. Both extracts showed appreciable anti-collagenase, anti-elastase, anti-hyaluronidase, and antioxidant activities, suggesting protective potential against extracellular matrix degradation and oxidative damage. Cytotoxicity assessment using LDH release assays confirmed concentration-dependent effects, and ultrastructural analyses using scanning and transmission electron microscopy revealed extract-induced morphological changes in dermal fibroblasts, including alterations in cell wall integrity, cytoplasmic organization, and intercellular structure. The results suggest that A. vasica and C. procera are promising sources of bioactive compounds with potential applications in dermatological formulations, including topical preparations to manage skin disorders and prevent enzymatic skin degradation.
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The Science of Nature - Naturwissenschaften - is Springer''s flagship multidisciplinary science journal. The journal is dedicated to the fast publication and global dissemination of high-quality research and invites papers, which are of interest to the broader community in the biological sciences. Contributions from the chemical, geological, and physical sciences are welcome if contributing to questions of general biological significance. Particularly welcomed are contributions that bridge between traditionally isolated areas and attempt to increase the conceptual understanding of systems and processes that demand an interdisciplinary approach.
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