PS12-2. Lipopolysaccharide (LPS) Challenge Alters Oxidative Stress Markers in Dairy-beef Breeds.

IF 3.7 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Journal of animal science Pub Date : 2026-09-29 DOI:10.1093/jas/skag272.348

Sam Barker, Joshua Slay, Mackenzie M Smithyman, Nicole C Burdick Sanchez, Paul R Broadway, Ryan R Rathmann, Michael A Ballou, Jerrad Legako

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引用次数: 0

Abstract

The objective of this study was to evaluate dairy cross-breeding influence on oxidative stress incurred when subjected to a lipopolysaccharide (LPS) challenge. Steers (n = 30; 194 kg ± 40 kg) were transported to the USDA- Livestock Issues Research Unit where they were housed in individual pens with ad libitum access to a receiving ration and water. Breed differences were established as crossbred beef steers (NAT; n = 9), Charolais × Jersey crossbred steers (CHAR; n = 10), and Holstein × Angus crossbred steers (HOL; n = 11). Steers were fitted with jugular catheters and indwelling rectal thermometers on d -1. On d 0, steers were challenged i.v. with LPS (0.15 μg/kg BW) and blood samples were collected at -2, 0, 1, 2, 4, 6, 8, 12, 24, 36, and 48 h relative to administration of LPS. Blood was further processed for plasma and analyzed for lipid oxidation via thiobarbituric reactive substance (TBARS) and total antioxidant capacity (TAC). Data were analyzed as a repeated measure using the GLIMMIX procedure of SAS. There was a breed effect on malondialdehye (MDA) concentrations via TBARS (P = 0.05), where concentrations were lowest in NAT steers when compared to HOL (P = 0.02) and CHAR (P = 0.06). There was a main effect of time on MDA (P < 0.001), where concentrations reached their lowest at 2 h post-challenge, but increased after 4 h (P < 0.001), where concentrations were then similar from 6 h until 36 h (P ≥ 0.05). From 36 to 48 h post-challenge, concentrations decrease again to levels similar to those at h 2 (P ≥ 0.12). An interaction occurred between breed type and time for TAC (P = 0.03). By 6 h, CHAR had the greatest TAC compared to NAT (P = 0.03) and HOL (P = 0.06). This continued to 8 h, where CHAR had a greater TAC compared to NAT (P = 0.04). These data suggest that breed type may influence the ability to manage oxidative stress during an immune challenge, which can have further impacts on feedlot performance, management practices, and meat quality as terminal breeds.

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PS12-2。脂多糖（LPS）挑战改变奶牛品种氧化应激标志物。

本研究的目的是评估奶牛杂交对脂多糖（LPS）胁迫下氧化应激的影响。阉牛（n = 30; 194 kg±40 kg）被运送到美国农业部牲畜问题研究单位，在那里它们被安置在单独的围栏中，可以自由地获得接收口粮和水。分别采用杂交肉牛（NAT, n = 9）、夏洛莱×泽西杂交肉牛（CHAR, n = 10）和荷斯坦×安格斯杂交肉牛（HOL, n = 11）建立品种差异。在第1天，用颈静脉导管和直肠内留置体温计。于第0天静脉注射LPS (0.15 μg/kg BW)，并于相对于LPS给药的第2、0、1、2、4、6、8、12、24、36和48 h采集血液样本。血液进一步处理为血浆，并通过硫代巴比妥反应物质（TBARS）和总抗氧化能力（TAC）分析脂质氧化。使用SAS的GLIMMIX程序对数据进行重复测量分析。TBARS对丙二醛（MDA）浓度有品种影响（P = 0.05），其中NAT组的MDA浓度低于HOL组（P = 0.02）和CHAR组（P = 0.06）。时间对MDA有主要影响（P < 0.001）， MDA浓度在攻毒后2 h达到最低，但在4 h后升高（P < 0.001），然后从6 h到36 h浓度相似（P≥0.05）。攻毒后36 ~ 48 h，浓度再次下降至与h 2相似的水平（P≥0.12）。品种类型与TAC时间之间存在交互作用（P = 0.03）。6 h时，CHAR组TAC高于NAT组（P = 0.03）和HOL组（P = 0.06）。这种情况持续到8小时，与NAT相比，CHAR具有更高的TAC （P = 0.04）。这些数据表明，品种类型可能会影响免疫挑战期间处理氧化应激的能力，这可能进一步影响作为终端品种的饲养场性能、管理实践和肉品质。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of animal science 农林科学-奶制品与动物科学

CiteScore

4.80

自引率

12.10%

发文量

1589

审稿时长

3 months

期刊介绍： The Journal of Animal Science (JAS) is the premier journal for animal science and serves as the leading source of new knowledge and perspective in this area. JAS publishes more than 500 fully reviewed research articles, invited reviews, technical notes, and letters to the editor each year. Articles published in JAS encompass a broad range of research topics in animal production and fundamental aspects of genetics, nutrition, physiology, and preparation and utilization of animal products. Articles typically report research with beef cattle, companion animals, goats, horses, pigs, and sheep; however, studies involving other farm animals, aquatic and wildlife species, and laboratory animal species that address fundamental questions related to livestock and companion animal biology will be considered for publication.