PS4-2. Triticale Silage Used as Ruminal in Situ Substrate to Assess the Effects of Saccharomyces Cerevisiae in Beef Cattle Grower and Finisher Diets.

IF 3.7 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Journal of animal science Pub Date : 2026-09-29 DOI:10.1093/jas/skag272.413

Fernanda Berumen, Jhones O Sarturi, Ahmari D Henry, Brooke A McCrumb, Kaliu Scaranto Silva, Kip K Karges, Beatriz Q Dos Reis, Osman R A Melgar, Otavio France

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Abstract

Ruminal degradability kinetics of triticale silage (in situ substrate) of beef steers consuming beef cattle grower and finisher diets with or without (live yeast) Saccharomyces cerevisiae were evaluated. Eight surgically prepared beef steers were used in a duplicated 4 × 4 Latin square design (four 38-day periods) following a 2 × 2 factorial arrangement of treatments: A) presence of live yeast (Saccharomyces cerevisiae [CNCM I-1077], at 1 × 1010 CFU/animal-daily); and B) diet type (steam-flaked corn-based grower or finisher diets [ad libitum intake]). Live yeast was delivered via gel capsules (0.25 g, as-is) twice daily through the ruminal cannula. Pre-dehydrated (55ºC for 72 h) triticale silage substrate was ground (2 mm) and placed into 10 × 20 cm (28 µm) nylon bags (5g, as-is). Bags were placed in the ruminal ventral sac within a nylon mesh (with weights) and reversely removed at 0, 2, 4, 8, 12, 20, 32, 48, 64, 72, and 96 h after incubation. Residue bags were rinsed and dehydrated for 72 h at 55ºC. Residue duplicates were composited, corrected for moisture, and used to fit a first-order kinetics model using the NLIN procedure of SAS. The GLIMMIX procedure of SAS was used with animals as the experimental unit, the fixed effects (diet, period, interaction), and the random effects of animal ID within square, square, and period. Live yeast inclusion tended (Diet × Yeast interaction, P = 0.06) to increase the rate of degradation and the effective degradability calculated at 4, 5, and 6 %/h of the substrate organic matter (OM) when offered in finisher diets, while not affecting such fractions in grower diets. Regardless of diet type, live yeast increased the substrate dry matter (DM) rate of degradation (P = 0.04), tended (P = 0.10) to increase the effective degradability of the DM fraction (calculated at 4%/h rate of passage), and tended (P = 0.11) to decrease the substrate hemicellulose fraction lag-time (h). Regardless of live yeast inclusion, steers offered the grower diet had greater (P < 0.01) triticale silage substrate potentially degradable fraction, lesser undegradable (P < 0.01), and greater (P < 0.01) effective degradability calculated at 4, 5, and 6 %/h rate of passage for DM, OM, NDF, ADF, and hemicellulose fractions, while also tending (P = 0.11) to increase the substrate DM rate of degradation compared to finisher diets. The current ruminal in situ model replicated expected results between beef cattle steam-flaked corn-based grower and finisher diets when using a forage substrate. Live yeast seemed to improve ruminal effective degradability of a high-quality forage substrate (triticale silage) OM fraction in a beef cattle finisher diet more intensively than in a grower diet, while other positive effects were observed regardless of diet type.

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PS4-2。用小黑麦青贮作为瘤胃原位底物评价酿酒酵母在肉牛种育饲料中的作用。

研究了食用含或不含（活酵母）酿酒酵母的肉牛生长和育肥牛日粮的肉牛小黑麦青贮（原位底物）的瘤胃降解动力学。8只手术准备的牛被用于重复的4 × 4拉丁方设计（4个38天周期），遵循2 × 2因子处理安排：a)存在活酵母（Saccharomyces cerevisiae [CNCM I-1077]， 1 × 1010 CFU/动物每日）；B)日粮类型（以蒸汽片玉米为基础的种植或育肥猪日粮[随意摄入]）。活酵母通过凝胶胶囊（0.25 g，原样）每天两次通过瘤胃插管输送。预脱水（55ºC， 72小时）的小黑麦青贮基质磨碎（2mm），放入10 × 20 cm（28µm）的尼龙袋（5g，原样）。将袋子放置在尼龙网（带重量）内的瘤胃腹囊中，并在孵育后0、2、4、8、12、20、32、48、64、72和96 h反向取出。将渣袋冲洗干净，在55℃下脱水72 h。利用SAS的NLIN程序，对残留重复物进行了合成、水分校正，并用于拟合一级动力学模型。采用SAS的GLIMMIX程序，以动物为实验单位，采用固定效应（日粮、周期、相互作用）和动物ID在平方、平方、周期内的随机效应。在育肥猪饲粮中添加活酵母（饲粮与酵母相互作用，P = 0.06），可提高降解率和底物有机物（OM）在4、5和6% /h时的有效降解率，而对生长猪饲粮中的有效降解率没有影响。无论饲粮类型如何，活酵母均提高了底物干物质（DM）的降解率（P = 0.04），有提高DM部分有效降解率（P = 0.10）的趋势（按4%/h传代率计算），有降低底物半纤维素部分滞后时间(h)的趋势（P = 0.11）。无论是否添加活酵母，按DM、OM、NDF、ADF和半纤维素的4、5和6% /h传代率计算，饲喂生长饲粮的阉牛小黑麦青贮底物潜在降解率更高（P < 0.01），不可降解率更低（P < 0.01），且与育肥饲粮相比，底物DM的降解率也更高（P < 0.01）， P = 0.11。目前的瘤胃原位模型在使用草料基质时复制了肉牛蒸汽片玉米生长日粮和育肥日粮之间的预期结果。与生长日粮相比，活酵母似乎更能提高肉牛育肥猪日粮中优质饲料基质（小黑麦青贮）OM部分的瘤胃有效降解率，而其他积极影响与日粮类型无关。

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来源期刊

Journal of animal science 农林科学-奶制品与动物科学

CiteScore

4.80

自引率

12.10%

发文量

1589

审稿时长

3 months

期刊介绍： The Journal of Animal Science (JAS) is the premier journal for animal science and serves as the leading source of new knowledge and perspective in this area. JAS publishes more than 500 fully reviewed research articles, invited reviews, technical notes, and letters to the editor each year. Articles published in JAS encompass a broad range of research topics in animal production and fundamental aspects of genetics, nutrition, physiology, and preparation and utilization of animal products. Articles typically report research with beef cattle, companion animals, goats, horses, pigs, and sheep; however, studies involving other farm animals, aquatic and wildlife species, and laboratory animal species that address fundamental questions related to livestock and companion animal biology will be considered for publication.