PS9-7. Proteomic Analysis Reveals Change in the Lipid Metabolism of Muscle in Beef Cattle Submitted to Early Weaning and Pasture Finished.

IF 3.7 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Journal of animal science Pub Date : 2026-09-29 DOI:10.1093/jas/skag272.515

Juliana A Torrecilhas, Gabriel L N Liberal, Gabriela B C Lima, Luis A L Chardulo, Philipe Moriel, Hugo Osório, Marcos E Buzanskas, André M Almeida, Irene Reis, Guilherme L Pereira

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引用次数: 0

Abstract

Early weaning and postnatal nutritional management are crucial strategies in livestock production systems and can affect long-term growth in beef cattle. This study aimed to evaluate the effect of early weaning associated with post-weaning supplementation on the muscle proteome of pasture-finished cattle. Forty male calves were assigned to early weaning (EW; 120 days, n = 20) or conventional weaning (CW; 205 days, n = 20). The EW group received protein–energy supplementation (20% CP; 78% TDN) at 10 g/kg BW/day until 205 days of age. After 205 days, all animals were raised together on pasture with protein–energy supplementation until slaughter (915 days). Longissimus thoracis muscle biopsies were collected at 205 and 915 days (pre-slaughter) and analyzed by nanoLC-MS/MS. The comparisons were as follows: (1) EW vs. CW at 205 days; (2) EW vs. CW at slaughter; (3) slaughter vs. 205 days in the EW group; and (4) slaughter vs. 205 days in the CW group. At 205 days, EW calves showed lower abundances of proteins related to lipid metabolism (CPT1B, SCP2, FABP3) and cellular stress (HSPB7), and higher abundances of proteins associated with muscle development (CSRP3, MYOZ2), compared to CW calves. At slaughter, fewer differences were observed between treatments, and the enriched pathways were primarily associated with oxidative phosphorylation; specifically, an upregulation of ATP5PF and a downregulation of NDUFB6 were observed in the EW group. Longitudinal analyses (205 days vs. slaughter) revealed distinct temporal trajectories in metabolic regulation between EW and CW groups. The transition to slaughter in both groups was characterized by the downregulation of proteins involved in mitochondrial lipid synthesis (HSD17B8) and β-oxidation (ACAA2, HADHA, HADHB, ACADS, ACADM, ACADVL, and ECI2). However, this decline was more pronounced in the CW group, which also showed a reduction in MDH2 and SDHB. Collectively, these findings suggest that the downregulation of mitochondrial oxidative metabolism at slaughter compared with 205 days was more pronounced in the CW group. An increased abundance of proteins related to lipid transport (APOD) and oxidative stress response (CFH, C3, C6, C7, and C9) was observed, along with elevated levels of complement system components. The EW group exhibited more balanced regulation, indicating improved energy efficiency and reduced inflammation throughout growth. Overall, early weaning with supplementation altered proteins related to lipid metabolism at 205 days, however, these differences were not maintained until slaughter. Funding from the São Paulo Research Foundation (FAPESP): 2022/10240-8, 2023/16301-1, and 2023/16127-1.

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PS9-7。蛋白质组学分析揭示了早期断奶和牧场育肥牛肌肉脂质代谢的变化。

早期断奶和产后营养管理是畜牧生产系统中的关键战略，可影响肉牛的长期生长。本研究旨在评估早期断奶与断奶后补充对放牧肉牛肌肉蛋白质组的影响。将40头雄性犊牛分为早期断奶组（EW， 120天，n = 20）和常规断奶组（CW， 205天，n = 20）。EW组以10 g/kg BW/天的速度补充蛋白质能量（20% CP, 78% TDN），直至205日龄。205 d后，所有动物在牧场上一起饲养，并补充蛋白质能量，直至屠宰（915 d）。于205和915天（屠宰前）采集胸最长肌活检，采用纳米lc -MS/MS进行分析。比较如下：(1)205 d EW与CW；(2) EW与CW在屠宰上的对比；(3)屠宰量vs. EW组205天；(4)屠宰，而CW组为205天。在第205天，与连续犊牛相比，EW犊牛的脂质代谢相关蛋白（CPT1B、SCP2、FABP3）和细胞应激相关蛋白（HSPB7）的丰度较低，而与肌肉发育相关蛋白（CSRP3、MYOZ2）的丰度较高。屠宰时，不同处理之间的差异较小，富集的途径主要与氧化磷酸化有关；具体来说，在EW组中观察到ATP5PF上调和NDUFB6下调。纵向分析（205天vs屠宰）揭示了EW组和CW组之间代谢调节的不同时间轨迹。在向屠宰过渡的过程中，两组小鼠线粒体脂质合成蛋白（HSD17B8）和β-氧化蛋白（ACAA2、HADHA、HADHB、ACADS、ACADM、ACADVL和ECI2）的表达均下调。然而，这种下降在CW组更为明显，MDH2和SDHB也有所减少。总的来说，这些发现表明，与205天相比，CW组在屠宰时线粒体氧化代谢的下调更为明显。观察到与脂质转运（APOD）和氧化应激反应（CFH, C3, C6， C7和C9）相关的蛋白质丰度增加，补体系统成分水平升高。EW组表现出更平衡的调节，表明在整个生长过程中能量效率提高，炎症减少。总体而言，在第205天早期断奶时，添加补充剂改变了与脂质代谢相关的蛋白质，然而，这些差异直到屠宰时才保持。圣保罗研究基金会（FAPESP）资助：2022/10240-8、2023/16301-1和2023/16127-1。

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来源期刊

Journal of animal science 农林科学-奶制品与动物科学

CiteScore

4.80

自引率

12.10%

发文量

1589

审稿时长

3 months

期刊介绍： The Journal of Animal Science (JAS) is the premier journal for animal science and serves as the leading source of new knowledge and perspective in this area. JAS publishes more than 500 fully reviewed research articles, invited reviews, technical notes, and letters to the editor each year. Articles published in JAS encompass a broad range of research topics in animal production and fundamental aspects of genetics, nutrition, physiology, and preparation and utilization of animal products. Articles typically report research with beef cattle, companion animals, goats, horses, pigs, and sheep; however, studies involving other farm animals, aquatic and wildlife species, and laboratory animal species that address fundamental questions related to livestock and companion animal biology will be considered for publication.