{"title":"Effects of Berberine chloride hydrate on cariogenic multispecies streptococcal biofilm.","authors":"Yang Zhou, Guxin Cui, Fang Huang, Huancai Lin","doi":"10.1080/20002297.2026.2732737","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Dental caries, a bacterial infectious disease related to biofilm, occurs due to an increase in the proportion of cariogenic bacteria over that of commensal bacteria. This study aimed to create an in vitro biofilm model consisting of <i>S. sanguinis</i>, <i>S. gordonii</i>, <i>and S. mutans</i> to study the effects of Berberine chloride hydrate (BH) on the structure and cariogenic features of multispecies biofilms.</p><p><strong>Materials and methods: </strong>The biofilm formation and cariogenic characteristics of multispecies streptococci biofilms were observed. The microbial composition of multispecies biofilms was quantified using fluorescent in situ hybridization (FISH). Besides, transcriptome analysis was performed to determine the differential expression levels of genes in each strain in multispecies biofilms with or without BH. The demineralization activity of biofilms treated with BH was analyzed using micro-CT.</p><p><strong>Results: </strong>BH had the highest efficacy against <i>S. mutans</i>, exhibiting lower minimum inhibitory concentration (MIC) and minimum biofilm inhibitory concentration (MBIC) values relative to <i>S. sanguinis</i> and <i>S. gordonii</i>. BH inhibited the biofilm formation as well as EPS synthesis in multispecies biofilms, resulting in decreased demineralization on bovine enamel surfaces. In addition, after BH treatment, the abundance of <i>S. sanguinis</i> and <i>S. mutans</i> was reduced, whereas the abundance of <i>S. gordonii</i> increased.</p><p><strong>Conclusion: </strong>BH revealed great potential for the management of cariogenic biofilm, indicating its possible application in dental caries therapy.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"18 1","pages":"2732737"},"PeriodicalIF":6.7000,"publicationDate":"2026-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13618191/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Oral Microbiology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1080/20002297.2026.2732737","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2026/1/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Background: Dental caries, a bacterial infectious disease related to biofilm, occurs due to an increase in the proportion of cariogenic bacteria over that of commensal bacteria. This study aimed to create an in vitro biofilm model consisting of S. sanguinis, S. gordonii, and S. mutans to study the effects of Berberine chloride hydrate (BH) on the structure and cariogenic features of multispecies biofilms.
Materials and methods: The biofilm formation and cariogenic characteristics of multispecies streptococci biofilms were observed. The microbial composition of multispecies biofilms was quantified using fluorescent in situ hybridization (FISH). Besides, transcriptome analysis was performed to determine the differential expression levels of genes in each strain in multispecies biofilms with or without BH. The demineralization activity of biofilms treated with BH was analyzed using micro-CT.
Results: BH had the highest efficacy against S. mutans, exhibiting lower minimum inhibitory concentration (MIC) and minimum biofilm inhibitory concentration (MBIC) values relative to S. sanguinis and S. gordonii. BH inhibited the biofilm formation as well as EPS synthesis in multispecies biofilms, resulting in decreased demineralization on bovine enamel surfaces. In addition, after BH treatment, the abundance of S. sanguinis and S. mutans was reduced, whereas the abundance of S. gordonii increased.
Conclusion: BH revealed great potential for the management of cariogenic biofilm, indicating its possible application in dental caries therapy.
期刊介绍：
As the first Open Access journal in its field, the Journal of Oral Microbiology aims to be an influential source of knowledge on the aetiological agents behind oral infectious diseases. The journal is an international forum for original research on all aspects of ''oral health''. Articles which seek to understand ''oral health'' through exploration of the pathogenesis, virulence, host-parasite interactions, and immunology of oral infections are of particular interest. However, the journal also welcomes work that addresses the global agenda of oral infectious diseases and articles that present new strategies for treatment and prevention or improvements to existing strategies.
Topics: ''oral health'', microbiome, genomics, host-pathogen interactions, oral infections, aetiologic agents, pathogenesis, molecular microbiology systemic diseases, ecology/environmental microbiology, treatment, diagnostics, epidemiology, basic oral microbiology, and taxonomy/systematics.
Article types: original articles, notes, review articles, mini-reviews and commentaries
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