Genetic polymorphisms in the SLC27A5 gene are associated with biochemical characteristics of HBV patients in Yunnan.

IF 1.9 4区医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL

Journal of applied biomedicine Pub Date : 2026-09-01 Epub Date: 2026-09-10 DOI:10.32725/jab.2026.008

Chaoqi Zhu, Qingyuan Wei, Yue Feng, Xueshan Xia, A- Mei Zhang

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引用次数: 0

Abstract

Background: Lipid droplets could influence hepatitis B virus (HBV) replication and release, as well as disease progression in patients with chronic HBV. The solute carrier family 27 member 5 (SLC27A5) gene is highly expressed in the liver and possessed bile acid-CoA ligase activity. However, no previous study has investigated the role of the SLC27A5 gene in HBV infection or disease progression.

Methods: A total of 400 chronic HBV patients and 300 controls were collected in Yunnan Province to genotype three single nucleotide polymorphisms (SNPs) of the SLC27A5 gene and investigate potential association between SNPs and HBV infection or biochemical characteristics of HBV patients. In silico prediction and dual-luciferase reporter assay were performed to explore the role of alleles of each SNP.

Results: No association was found between HBV infection and genotypes and alleles of three SNPs (rs4801275, rs2278497, and rs56225452) in the SLC27A5 gene. However, alanine transaminase (ALT) and lymphocytes (LYM) levels differed significantly among HBV patients carrying different genotypes of rs4801275. When HBV patients were stratified into HBsAg-positive and HBsAg-negative subgroups, most hepatic functional features showed significant differences between the two subgroups. Furthermore, RNA structures containing the alleles of two SNPs were predicted, and the results indicated that different alleles could partially alter RNA structures. Dual-luciferase reporter assay also demonstrated that different alleles of the three SNPs significantly influenced firefly luciferase expression level.

Conclusion: This study is the first to identify that genetic polymorphisms of the SLC27A5 gene are associated with biochemical characteristics of HBV patients in Yunnan. Furthermore, the RNA structure prediction and dual-luciferase reporter assays suggest that different alleles of the three SNPs might alter the SLC27A5 gene expression levels.

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SLC27A5基因的遗传多态性与云南HBV患者的生化特征相关。

背景：脂滴可以影响乙型肝炎病毒（HBV）的复制和释放，以及慢性HBV患者的疾病进展。溶质载体家族27成员5 （SLC27A5）基因在肝脏中高度表达，具有胆汁酸-辅酶a连接酶活性。然而，之前没有研究调查SLC27A5基因在HBV感染或疾病进展中的作用。方法：收集云南省400例慢性HBV患者和300例对照者，对SLC27A5基因的3个单核苷酸多态性（snp）进行基因分型，探讨snp与HBV感染或HBV患者生化特征的潜在关联。通过计算机预测和双荧光素酶报告基因分析来探讨每个SNP等位基因的作用。结果：HBV感染与SLC27A5基因3个snp （rs4801275、rs2278497和rss56225452）基因型和等位基因无相关性。然而，携带不同rs4801275基因型的HBV患者的谷丙转氨酶（ALT）和淋巴细胞（LYM）水平存在显著差异。当将HBV患者分为hbsag阳性和hbsag阴性亚组时，大多数肝功能特征在两个亚组之间显示出显著差异。此外，对含有两个snp等位基因的RNA结构进行了预测，结果表明不同的等位基因可以部分改变RNA结构。双荧光素酶报告基因实验也表明，三个snp的不同等位基因显著影响萤火虫荧光素酶的表达水平。结论：本研究首次发现SLC27A5基因的遗传多态性与云南地区HBV患者的生化特征相关。此外，RNA结构预测和双荧光素酶报告基因分析表明，三个snp的不同等位基因可能改变SLC27A5基因的表达水平。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of applied biomedicine PHARMACOLOGY & PHARMACY-

CiteScore

2.40

自引率

7.70%

发文量

审稿时长

>12 weeks

期刊介绍： Journal of Applied Biomedicine promotes translation of basic biomedical research into clinical investigation, conversion of clinical evidence into practice in all medical fields, and publication of new ideas for conquering human health problems across disciplines. Providing a unique perspective, this international journal publishes peer-reviewed original papers and reviews offering a sensible transfer of basic research to applied clinical medicine. Journal of Applied Biomedicine covers the latest developments in various fields of biomedicine with special attention to cardiology and cardiovascular diseases, genetics, immunology, environmental health, toxicology, neurology and oncology as well as multidisciplinary studies. The views of experts on current advances in nanotechnology and molecular/cell biology will be also considered for publication as long as they have a direct clinical impact on human health. The journal does not accept basic science research or research without significant clinical implications. Manuscripts with innovative ideas and approaches that bridge different fields and show clear perspectives for clinical applications are considered with top priority.