Genomic characterization of the first goat-associated orf virus genome sequenced directly from a human clinical sample in Portugal

IF 2.5 4区医学 Q3 VIROLOGY

Archives of Virology Pub Date : 2026-09-28 DOI:10.1007/s00705-026-06749-4

Daniel Sobral, Rita Cordeiro, Ana Paula Brito, Ana Margarida Henriques, Sílvia C. Barros, Fábio Abade dos Santos, Margarida D. Duarte, Ana Pelerito, Isabel Lopes de Carvalho, Luís Coelho, Rita Ferreira, Sara Rangel, Luís Vieira, Maria Sofia Núncio, Vítor Borges, João Paulo Gomes

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Abstract

The orf virus (ORFV) is a neglected zoonotic virus, with sporadic human infections, historically identified through clinical suspicion rather than molecular confirmation. We report the first molecularly confirmed human ORFV case in Portugal and the first goat-associated human-derived ORFV genome. Direct hybrid capture sequencing of a clinical biopsy from a veterinary student bitten by a goat yielded a 129.5 kb genome. Phylogenetic analysis placed the isolate within a goat-associated cluster, distinct from the two previously available human-derived genomes. This case highlights the value of molecular surveillance and genomic sequencing for One Health surveillance of neglected zoonoses.

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直接从葡萄牙人类临床样本中测序的首个山羊相关口蹄疫病毒基因组的基因组特征

口蹄疫病毒（ORFV）是一种被忽视的人畜共患病毒，具有散发的人间感染，历史上是通过临床怀疑而不是分子证实发现的。我们报告了葡萄牙第一例分子确诊的人类ORFV病例和第一个与山羊相关的人类衍生ORFV基因组。对一名被山羊咬伤的兽医学生的临床活检进行直接杂交捕获测序，获得了129.5 kb的基因组。系统发育分析将该分离物置于山羊相关簇中，不同于先前可获得的两个人类衍生基因组。该病例强调了分子监测和基因组测序对One Health监测被忽视的人畜共患病的价值。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Archives of Virology 医学-病毒学

CiteScore

5.10

自引率

7.40%

发文量

324

审稿时长

4.5 months

期刊介绍： Archives of Virology publishes original contributions from all branches of research on viruses, virus-like agents, and virus infections of humans, animals, plants, insects, and bacteria. Coverage spans a broad spectrum of topics, from descriptions of newly discovered viruses, to studies of virus structure, composition, and genetics, to studies of virus interactions with host cells, organisms and populations. Studies employ molecular biologic, molecular genetics, and current immunologic and epidemiologic approaches. Contents include studies on the molecular pathogenesis, pathophysiology, and genetics of virus infections in individual hosts, and studies on the molecular epidemiology of virus infections in populations. Also included are studies involving applied research such as diagnostic technology development, monoclonal antibody panel development, vaccine development, and antiviral drug development.Archives of Virology wishes to publish obituaries of recently deceased well-known virologists and leading figures in virology.