Bioactive Zein–Nondigestible Oligosaccharide Nanofibers Enable Rapid Tissue Regeneration and Antibacterial Defense

IF 6 2区医学 Q2 MATERIALS SCIENCE, BIOMATERIALS

ACS Biomaterials Science & Engineering Pub Date : 2026-10-12 Epub Date: 2026-09-29 DOI:10.1021/acsbiomaterials.6c00314

Renugaa Suresh Babu, Piotr Gierlich, Ramakrishnan Swaminathan, Vimalraj Selvaraj, Wendy W. J. Unger, Swathi Sudhakar

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引用次数: 0

Abstract

Chronic wounds pose a significant clinical challenge due to persistent inflammation, microbial infection, and impaired tissue regeneration. Conventional wound dressings often lack the multifunctionality needed for comprehensive wound management. In this study, we engineered a nanofibrous wound patch composed of zein (ZM) integrated with nondigestible oligosaccharides (ZM-NDOs), such as Chito-oligosaccharides (ZMC), guluronic and mannuronic-rich alginate-oligosaccharides (ZMAGM), and guluronic-rich alginate-oligosaccharides (ZMAG), to address these limitations. Structural and physicochemical analyses (SEM, FTIR, UTM, and water contact angle) confirmed the formation of bead-free, interconnected fibers with enhanced mechanical strength and hydrophilicity. In vitro cytocompatibility assays using HEK 293T cells demonstrated excellent biocompatibility for both ZM and ZM-NDO patches. Notably, ZMC significantly promoted cellular proliferation and migration in wound scratch assays, accelerating wound closure. In vivo evaluation using a Wistar rat excision wound model further substantiated the therapeutic efficacy of the ZM-NDO patches, showing improved collagen deposition, vascularization, and epithelial regeneration, accompanied by upregulated expression of VEGF, TGF-β, and Col-I mRNA. Moreover, the ZMC scaffolds exhibited significant antibacterial activity against Pseudomonas aeruginosa and methicillin-resistant Staphylococcus aureus. Collectively, these findings highlight ZM-NDO, particularly ZMC, as a promising, multifunctional, and scalable platform for advanced wound-healing applications.

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生物活性玉米蛋白-难消化低聚糖纳米纤维促进快速组织再生和抗菌防御。

由于持续的炎症、微生物感染和组织再生受损，慢性伤口构成了重大的临床挑战。传统的伤口敷料往往缺乏综合伤口管理所需的多功能。在这项研究中，我们设计了一种纳米纤维伤口贴片，由玉米蛋白（ZM）与不可消化低聚糖（ZM- ndos），如壳寡糖（ZMC）、富含古鲁醛酸和甘露醛酸的海藻酸低聚糖（ZMAGM）和富含古鲁醛酸的海藻酸低聚糖（ZMAG）整合而成，以解决这些限制。结构和物理化学分析（SEM， FTIR， UTM和水接触角）证实形成了无珠，相互连接的纤维，具有增强的机械强度和亲水性。体外细胞相容性实验表明，HEK 293T细胞对ZM和ZM- ndo贴片具有良好的生物相容性。值得注意的是，在伤口划伤实验中，ZMC显著促进细胞增殖和迁移，加速伤口愈合。Wistar大鼠切除伤口模型的体内评价进一步证实了ZM-NDO贴片的治疗效果，显示出胶原沉积、血管形成和上皮再生的改善，并伴有VEGF、TGF-β和col - 1 mRNA的上调表达。此外，ZMC支架对铜绿假单胞菌和耐甲氧西林金黄色葡萄球菌具有明显的抗菌活性。总的来说，这些发现突出了ZM-NDO，特别是ZMC，作为一个有前途的，多功能的，可扩展的先进伤口愈合应用平台。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

ACS Biomaterials Science & Engineering Materials Science-Biomaterials

CiteScore

10.30

自引率

3.40%

发文量

413

期刊介绍： ACS Biomaterials Science & Engineering is the leading journal in the field of biomaterials, serving as an international forum for publishing cutting-edge research and innovative ideas on a broad range of topics: Applications and Health – implantable tissues and devices, prosthesis, health risks, toxicology Bio-interactions and Bio-compatibility – material-biology interactions, chemical/morphological/structural communication, mechanobiology, signaling and biological responses, immuno-engineering, calcification, coatings, corrosion and degradation of biomaterials and devices, biophysical regulation of cell functions Characterization, Synthesis, and Modification – new biomaterials, bioinspired and biomimetic approaches to biomaterials, exploiting structural hierarchy and architectural control, combinatorial strategies for biomaterials discovery, genetic biomaterials design, synthetic biology, new composite systems, bionics, polymer synthesis Controlled Release and Delivery Systems – biomaterial-based drug and gene delivery, bio-responsive delivery of regulatory molecules, pharmaceutical engineering Healthcare Advances – clinical translation, regulatory issues, patient safety, emerging trends Imaging and Diagnostics – imaging agents and probes, theranostics, biosensors, monitoring Manufacturing and Technology – 3D printing, inks, organ-on-a-chip, bioreactor/perfusion systems, microdevices, BioMEMS, optics and electronics interfaces with biomaterials, systems integration Modeling and Informatics Tools – scaling methods to guide biomaterial design, predictive algorithms for structure-function, biomechanics, integrating bioinformatics with biomaterials discovery, metabolomics in the context of biomaterials Tissue Engineering and Regenerative Medicine – basic and applied studies, cell therapies, scaffolds, vascularization, bioartificial organs, transplantation and functionality, cellular agriculture