{"title":"Reverse Breeding: A Breakthrough Technique for Genetic Fixation and Hybrid Reconstruction","authors":"Manoj Kumar Patel, Vijay Kamal Meena, Sanjay Kumar, Pawan Kumar, Subhash Chand, Ankit Malik, Kadam Kiran, Bharath Kumar Alam, Arjun Lal Ola, Girija Choudhary, Rakesh Choudhary","doi":"10.1002/dvg.70070","DOIUrl":null,"url":null,"abstract":"<div>\n \n <p>Reverse breeding (RB), a novel plant breeding approach, helps to identify the parental homozygous lines for any uncharacterized but useful heterozygous plant that a plant breeder needs utmost. For this, genetic engineering at meiotic cell division, particularly meiosis-I, must achieve the right combination and complement homozygous parental lines for recreating the desired hybrid. In this technology, meiotic cross-over is suppressed to stop the recombination at the chromosome level in the selected heterozygous plants. The success of RB in crops depends on the efficiency and effectiveness of doubled haploid (DH) technology, which is needed to produce diploid (2<i>n</i>) plants from haploid gametes (<i>n</i>) after diploidization. The review initially highlights the current status and requirement of RB in addressing global challenges related to food and nutritional security. It then discusses the different mechanisms to engineer meiosis to suppress cross-over and the fixation of nonrecombinant chromosomes through DH technology. The review further examines immediate applications of RB and the factors influencing its efficiency. The advantages and limitations of RB are also discussed, along with its potential applications in future crop improvement. In conclusion, RB represents a promising plant breeding approach for overcoming prevailing challenges through the development of crop varieties with improved disease resistance, climate resilience, nutritional quality, and other traits relevant to sustainable agriculture and human needs.</p>\n </div>","PeriodicalId":12718,"journal":{"name":"genesis","volume":"64 5","pages":""},"PeriodicalIF":2.8000,"publicationDate":"2026-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"genesis","FirstCategoryId":"99","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/dvg.70070","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"DEVELOPMENTAL BIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Reverse breeding (RB), a novel plant breeding approach, helps to identify the parental homozygous lines for any uncharacterized but useful heterozygous plant that a plant breeder needs utmost. For this, genetic engineering at meiotic cell division, particularly meiosis-I, must achieve the right combination and complement homozygous parental lines for recreating the desired hybrid. In this technology, meiotic cross-over is suppressed to stop the recombination at the chromosome level in the selected heterozygous plants. The success of RB in crops depends on the efficiency and effectiveness of doubled haploid (DH) technology, which is needed to produce diploid (2n) plants from haploid gametes (n) after diploidization. The review initially highlights the current status and requirement of RB in addressing global challenges related to food and nutritional security. It then discusses the different mechanisms to engineer meiosis to suppress cross-over and the fixation of nonrecombinant chromosomes through DH technology. The review further examines immediate applications of RB and the factors influencing its efficiency. The advantages and limitations of RB are also discussed, along with its potential applications in future crop improvement. In conclusion, RB represents a promising plant breeding approach for overcoming prevailing challenges through the development of crop varieties with improved disease resistance, climate resilience, nutritional quality, and other traits relevant to sustainable agriculture and human needs.
期刊介绍：
As of January 2000, Developmental Genetics was renamed and relaunched as genesis: The Journal of Genetics and Development, with a new scope and Editorial Board. The journal focuses on work that addresses the genetics of development and the fundamental mechanisms of embryological processes in animals and plants. With increased awareness of the interplay between genetics and evolutionary change, particularly during developmental processes, we encourage submission of manuscripts from all ecological niches. The expanded numbers of genomes for which sequencing is being completed will facilitate genetic and genomic examination of developmental issues, even if the model system does not fit the “classical genetic” mold. Therefore, we encourage submission of manuscripts from all species. Other areas of particular interest include: 1) the roles of epigenetics, microRNAs and environment on developmental processes; 2) genome-wide studies; 3) novel imaging techniques for the study of gene expression and cellular function; 4) comparative genetics and genomics and 5) animal models of human genetic and developmental disorders.
genesis presents reviews, full research articles, short research letters, and state-of-the-art technology reports that promote an understanding of the function of genes and the roles they play in complex developmental processes.
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