Bioconversion of agricultural waste to ε-poly-L-lysine by an engineered Streptomyces albulus strain.

IF 4.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

World journal of microbiology & biotechnology Pub Date : 2026-09-27 DOI:10.1007/s11274-026-05283-4

Rui Huang, Ziling Yuan, Yujie Liu, Liwen Zhang, Guanhua Li, Ruihua Liu, Chao Yang

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Abstract

The vast majority of agricultural wastes are burned or buried in soil currently, causing environmental pollution and resource waste worldwide. To achieve the eco-friendly bioconversion of agricultural waste, this study utilized an engineered Streptomyces albulus for solid-state fermentation (SSF) to convert rapeseed cake into ε-poly-L-lysine (ε-PL), the L-lysine homopolymer widely applied as a natural and safe food preservative. Firstly, based on genome analysis and the property characterization of agricultural waste, the bioconversion potential of S. albulus toward lignocellulosic waste was revealed, followed by preliminary experimental validation. Secondly, the high-titer engineered strain was obtained by overexpressing key genes eno1, eno2, pepc, ask and pls of ε-PL biosynthesis pathway, thereby promoting the bioconversion of rapeseed cake. Among the mutants, S. albulus PLS got the highest ε-PL titer, which was 9.3-fold that of the control strain in SSF. And the ε-PL titer was further increased to 1.6 times the original via optimizing SSF process. Finally, we characterized the growth and development status and the lignocellulose biodegradation of S. albulus PLS during the bioconversion process. Overall, this study identified a strategy for the valorization of agricultural waste, and the engineered strain offers a promising SSF microbial chassis for ε-PL production from lignocellulosic waste.

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农业废弃物转化为ε-聚l -赖氨酸的白色链霉菌工程菌株。

目前，绝大多数农业废弃物被焚烧或填埋在土壤中，造成了世界范围内的环境污染和资源浪费。为了实现农业废弃物的环保生物转化，本研究利用转基因白链霉菌固态发酵（SSF）将菜籽饼转化为ε-聚l -赖氨酸（ε-PL），这种l -赖氨酸均聚物是一种天然安全的食品防腐剂。首先，基于基因组分析和农业废弃物的特性表征，揭示了白葡萄球菌对木质纤维素废弃物的生物转化潜力，并进行了初步的实验验证。其次，通过过表达ε-PL生物合成途径的关键基因ene1、eno2、pepc、ask和pls，获得高滴度的工程菌株，从而促进油菜籽饼的生物转化。突变体中，白丝霉PLS的ε-PL效价最高，为对照菌株的9.3倍。通过优化SSF工艺，将ε-PL效价进一步提高到原来的1.6倍。最后，我们研究了白球藻PLS在生物转化过程中的生长发育状况和木质纤维素的生物降解。总的来说，本研究确定了农业废弃物的增值策略，并且工程菌株为从木质纤维素废弃物中生产ε-PL提供了一个有前途的SSF微生物底盘。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

World journal of microbiology & biotechnology 工程技术-生物工程与应用微生物

CiteScore

6.30

自引率

2.40%

发文量

257

审稿时长

2.5 months

期刊介绍： World Journal of Microbiology and Biotechnology publishes research papers and review articles on all aspects of Microbiology and Microbial Biotechnology. Since its foundation, the Journal has provided a forum for research work directed toward finding microbiological and biotechnological solutions to global problems. As many of these problems, including crop productivity, public health and waste management, have major impacts in the developing world, the Journal especially reports on advances for and from developing regions. Some topics are not within the scope of the Journal. Please do not submit your manuscript if it falls into one of the following categories: · Virology · Simple isolation of microbes from local sources · Simple descriptions of an environment or reports on a procedure · Veterinary, agricultural and clinical topics in which the main focus is not on a microorganism · Data reporting on host response to microbes · Optimization of a procedure · Description of the biological effects of not fully identified compounds or undefined extracts of natural origin · Data on not fully purified enzymes or procedures in which they are applied All articles published in the Journal are independently refereed.