[Diagnostic Value of PD-1 in Distinguishing the Aberrant T Cells from Angioimmunoblastic T-cell Lymphoma and Lymphoid Variant of Hypereosinophilic Syndrome].

Abstract

Objective: To explore the diagnostic significance of programmed death-1 (PD-1) expression in angioimmunoblastic T-cell lymphoma (AITL) and lymphoid variant of hypereosinophilic syndrome (L-HES).

Methods: Flow cytometry was used to detect aberrant CD3^-CD4 ⁺ lymphocytes in bone marrow samples from 35 AITL and 13 L-HES patients, the level of PD-1 expression on these cells was analyzed, and the positivity rate was calculated. CD3 ⁺CD4 ⁺ lymphocytes of healthy volunteers were utilized as normal controls, the difference in PD-1 expression between CD3 ⁺CD4 ⁺ lymphocytes in normal controls and aberrant CD3^- CD4 ⁺ lymphocytes in patients was compared.

Results: The positive rate of PD-1 expression in CD3^-CD4 ⁺ lymphocytes was significantly higher in the AITL group than that in the L-HES and normal control groups (both P <0.05), while there was no difference between L-HES group and normal control group (P >0.05). No significant difference in the positive rate of PD-1 expression between EBV- DNA ⁺ and EBV-DNA ^-patients in AITL group was observed (P >0.05).

Conclusion: The level of PD-1 expression detected by flow cytometry can help distinguish AITL from L-HES.