Thymidine kinase 1 activity in serum and body cavity fluids of horses with lymphoma.

IF 2.2 2区农林科学

Journal of Veterinary Internal Medicine Pub Date : 2026-09-01 DOI:10.1093/jvimsj/aalag207

Karolina Drozdzewska, Sophie Burde, Elisabeth Müller, Roswitha Merle, Heidrun Gehlen

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引用次数: 0

Abstract

Background: The accuracy of serum thymidine kinase 1 (TK1S) as a biomarker of lymphoma in horses is variable. The value of measurement in body cavity fluid (TK1F) has not been established.

Hypothesis/objectives: (1) TK1F and TK1S will be higher in horses with lymphoma compared to diseased controls (DCs); (2) TK1F will be higher than TK1S; and (3) there will be a strong correlation between both measurements.

Animals: Twenty animals in the lymphoma and 31 animals with nonneoplastic, gastrointestinal/respiratory disorders in DC group.

Methods: This was a cross-sectional study using prospectively collected data and samples obtained during routine diagnostic evaluation at referral hospitals. Thymidine kinase 1 was measured using chemiluminescence immunoassay and presented as median (and interquartile range). Nonparametric tests compared TK1F and TK1S between and within groups and Kendall's Tau assessed correlation between TK1F and TK1S.

Results: Median TK1F was 7.48 (2.20-48.23) U/L in horses with lymphoma vs 2.50 (0.59-4.83) U/L in the DC group (P = .007); median TK1S was 4.26 (0.46-9.37) U/L vs 0.40 (0.40-0.75) U/L (P < .001), respectively. Both biomarkers showed good discrimination (area under the curve > 0.70) between lymphoma and the DC group, with optimal cutoff values of 5.7 and 2.5 U/L, respectively. Thymidine kinase 1 activity in body cavity fluid (3.62 [0.85-9.86] U/L) was significantly higher than TK1S (0.50 [0.40-2.09] U/L; P < .001) and there was no significant correlation between them within groups.

Conclusions and clinical importance: Thymidine kinase 1 activity in body cavity fluid and TK1S provide different information and should be interpreted independently. They proved useful in lymphoma diagnosis, but not as a standalone test.

Abstract translated to german:

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淋巴瘤马血清和体腔液胸苷激酶1的活性。

背景：血清胸苷激酶1 （TK1S）作为马淋巴瘤的生物标志物的准确性是可变的。体腔液测量值（TK1F）尚未确定。假设/目的：(1)与患病对照（DCs）相比，淋巴瘤马的TK1F和TK1S更高；(2) TK1F将高于TK1S；(3)两种测量结果之间存在很强的相关性。动物：DC组淋巴瘤动物20只，非肿瘤性、胃肠道/呼吸系统疾病动物31只。方法：这是一项横断面研究，使用在转诊医院常规诊断评估中获得的前瞻性收集数据和样本。胸苷激酶1用化学发光免疫分析法测定，并以中位数（和四分位数范围）表示。非参数检验比较组间和组内TK1F和TK1S， Kendall's Tau评估TK1F和TK1S之间的相关性。结果：淋巴瘤组中位TK1F为7.48 (2.20-48.23)U/L， DC组中位TK1F为2.50 (0.59-4.83)U/L (P = .007)；淋巴瘤组和DC组的中位TK1S分别为4.26 (0.46-9.37)U/L和0.40 (0.40-0.75)U/L (P 0.70)，最佳截断值分别为5.7和2.5 U/L。腹腔液胸苷激酶1活性（3.62 [0.85-9.86]U/L）显著高于TK1S (0.50 [0.40-2.09] U/L)； P结论及临床意义：胸腔液胸苷激酶1活性与TK1S提供的信息不同，应独立解释。它们被证明在淋巴瘤诊断中是有用的，但不是作为一个独立的测试。摘要翻译成德语：

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Veterinary Internal Medicine Veterinary-General Veterinary

自引率

11.50%

发文量

243

期刊介绍： The mission of the Journal of Veterinary Internal Medicine is to advance veterinary medical knowledge and improve the lives of animals by publication of authoritative scientific articles of animal diseases.