Dual-Mode Fluorescence/UV-Vis Sensing Platform Based on Luminescence Modulation of Upconversion Nanoparticles.

IF 3.4 4区化学 Q2 BIOCHEMICAL RESEARCH METHODS

Journal of Fluorescence Pub Date : 2026-11-01 Epub Date: 2026-09-26 DOI:10.1007/s10895-026-04951-w

Leila Khaleghi, Nahid Sarlak

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引用次数: 0

Abstract

Upconverting nanoparticles (UCNPs) composed of sodium yttrium fluoride (NaYF₄) nanocrystals doped with trivalent rare-earth ions are synthesized by a co-precipitation method. Under 980 nm excitation, the UCNPs exhibit two prominent emission bands, both of which undergo significant quenching upon introduction of lycopene. Laser-induced fluorescence measurements reveal a pronounced decrease in luminescence intensity following lycopene addition. UV-visible absorption and steady-state fluorescence spectroscopy further demonstrate a linear correlation between lycopene concentration and signal attenuation. The limit of detection (LOD) for the UV-Vis assay is 328 ppm, while fluorescence detection attains an LOD of 234.5 ppm. Correlation coefficients (R²) of 0.9931 (UV-Vis) and 0.9958 (fluorescence) and relative standard deviations (RSD) of 3.9% and 1.76%, respectively, indicate high analytical performance. Quenching behavior is rationalized in terms of energy transfer mechanisms, underscoring the potential of UCNP-lycopene systems for optical sensing applications.

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基于上转换纳米粒子发光调制的双模荧光/紫外可见传感平台。

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来源期刊

Journal of Fluorescence 化学-分析化学

CiteScore

4.60

自引率

7.40%

发文量

203

审稿时长

5.4 months

期刊介绍： Journal of Fluorescence is an international forum for the publication of peer-reviewed original articles that advance the practice of this established spectroscopic technique. Topics covered include advances in theory/and or data analysis, studies of the photophysics of aromatic molecules, solvent, and environmental effects, development of stationary or time-resolved measurements, advances in fluorescence microscopy, imaging, photobleaching/recovery measurements, and/or phosphorescence for studies of cell biology, chemical biology and the advanced uses of fluorescence in flow cytometry/analysis, immunology, high throughput screening/drug discovery, DNA sequencing/arrays, genomics and proteomics. Typical applications might include studies of macromolecular dynamics and conformation, intracellular chemistry, and gene expression. The journal also publishes papers that describe the synthesis and characterization of new fluorophores, particularly those displaying unique sensitivities and/or optical properties. In addition to original articles, the Journal also publishes reviews, rapid communications, short communications, letters to the editor, topical news articles, and technical and design notes.