Generation of Vascular Organoids from Human iPSCs to Study Neurodegenerative Processes.

IF 0.7 4区医学 Q4 MEDICINE, RESEARCH & EXPERIMENTAL

Bulletin of Experimental Biology and Medicine Pub Date : 2026-05-01 Epub Date: 2026-09-26 DOI:10.1007/s10517-026-06736-4

D A Lifanov, E S Perepelitsa, A A Kopylova, A V Blagova, K O Salina, A Satish, S O Yurchenko, A B Salmina, S N Illarioshkin

引用次数: 0

Abstract

Organoids are a type of three-dimensional cellular constructs comprising cells of various specializations (phenotypes). In contrast to simple cellular spheroids (aggregates), cells within organoids exhibit more complex differentiation and functional characteristics. This study describes a protocol for generating vascular organoids incorporating vascular endothelial cells. A step-by-step workflow is presented, detailing the necessary reagents and consumables. Additionally, a method for validating differentiation outcomes using immunofluorescence staining is described. Morphological differences are reported between organoids derived from stem cells of a healthy donor and those from patients with Alzheimer's or Parkinson's disease.

查看原文本刊更多论文

利用人多能干细胞生成血管类器官研究神经退行性过程。

类器官是一种由各种特化（表型）细胞组成的三维细胞结构。与简单的细胞球体（聚集体）相比，类器官内的细胞表现出更复杂的分化和功能特征。本研究描述了一种结合血管内皮细胞生成血管类器官的方案。介绍了一步一步的工作流程，详细说明了必要的试剂和耗材。另外，描述了一种使用免疫荧光染色验证分化结果的方法。据报道，来自健康供体的干细胞和来自阿尔茨海默氏症或帕金森病患者的干细胞的类器官在形态学上存在差异。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Bulletin of Experimental Biology and Medicine 医学-医学：研究与实验

CiteScore

1.50

自引率

14.30%

发文量

265

审稿时长

2 months

期刊介绍： Bulletin of Experimental Biology and Medicine presents original peer reviewed research papers and brief reports on priority new research results in physiology, biochemistry, biophysics, pharmacology, immunology, microbiology, genetics, oncology, etc. Novel trends in science are covered in new sections of the journal - Biogerontology and Human Ecology - that first appeared in 2005. World scientific interest in stem cells prompted inclusion into Bulletin of Experimental Biology and Medicine a quarterly scientific journal Cell Technologies in Biology and Medicine (a new Russian Academy of Medical Sciences publication since 2005). It publishes only original papers from the leading research institutions on molecular biology of stem and progenitor cells, stem cell as the basis of gene therapy, molecular language of cell-to-cell communication, cytokines, chemokines, growth and other factors, pilot projects on clinical use of stem and progenitor cells. The Russian Volume Year is published in English from April.