Xiangcheng Lei, Ruipeng Li, Yangyi Nie, Deju Gao, Wei Zhang, Yuxiao Lai, Cairong Li
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引用次数: 0
Abstract
Magnesium ions (Mg2+) play pivotal roles in bone regeneration by regulating the biological functions of multiple bone repair-related cells. However, the effective concentration ranges and optimal concentration of Mg2+ for different cell types remain incompletely defined, which limits the precise design of Mg-containing biomaterials with controllable ion release for repairing of bone defects. In this study, the concentration-dependent effects of Mg2+ on the biological functions of bone regeneration-related cells were systematically investigated. Human bone marrow mesenchymal stem cells (hBMSCs), human umbilical vein endothelial cells (HUVECs), rat Schwann cells (RSCs), and RAW264.7 macrophage were selected to evaluate the effective concentration of Mg2+ for promoting osteogenesis, angiogenesis, neuroregulation, and immunomodulation, respectively. The results demonstrated that Mg2+ concentrations ranging from to 16 mM, particularly 8 mM, significantly upregulated the expression of M2 macrophage-related markers, thereby promoting the polarization of RAW264.7 macrophages toward the anti-inflammatory M2 phenotype. Moreover, Mg2+ concentrations between 2 and 8 mM effectively enhanced the osteogenic activity of hBMSCs, the angiogenic activity of HUVECs, and the neuroregulatory function of RSCs, as confirmed by immunofluorescence staining and quantitative PCR analysis of osteogenic, angiogenic, and neuroregulation-related marker expression. These findings provide a comprehensive understanding of the concentration-dependent biological effects of Mg2+ on bone regeneration-related cells and establish a theoretical basis for the rational design of next-generation Mg2+-releasing biomaterials with precise biological regulation.
期刊介绍：
Aims
Bioengineering (ISSN 2306-5354) provides an advanced forum for the science and technology of bioengineering. It publishes original research papers, comprehensive reviews, communications and case reports. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. All aspects of bioengineering are welcomed from theoretical concepts to education and applications. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. There are, in addition, four key features of this Journal:
● We are introducing a new concept in scientific and technical publications “The Translational Case Report in Bioengineering”. It is a descriptive explanatory analysis of a transformative or translational event. Understanding that the goal of bioengineering scholarship is to advance towards a transformative or clinical solution to an identified transformative/clinical need, the translational case report is used to explore causation in order to find underlying principles that may guide other similar transformative/translational undertakings.
● Manuscripts regarding research proposals and research ideas will be particularly welcomed.
● Electronic files and software regarding the full details of the calculation and experimental procedure, if unable to be published in a normal way, can be deposited as supplementary material.
● We also accept manuscripts communicating to a broader audience with regard to research projects financed with public funds.
Scope
● Bionics and biological cybernetics: implantology; bio–abio interfaces
● Bioelectronics: wearable electronics; implantable electronics; “more than Moore” electronics; bioelectronics devices
● Bioprocess and biosystems engineering and applications: bioprocess design; biocatalysis; bioseparation and bioreactors; bioinformatics; bioenergy; etc.
● Biomolecular, cellular and tissue engineering and applications: tissue engineering; chromosome engineering; embryo engineering; cellular, molecular and synthetic biology; metabolic engineering; bio-nanotechnology; micro/nano technologies; genetic engineering; transgenic technology
● Biomedical engineering and applications: biomechatronics; biomedical electronics; biomechanics; biomaterials; biomimetics; biomedical diagnostics; biomedical therapy; biomedical devices; sensors and circuits; biomedical imaging and medical information systems; implants and regenerative medicine; neurotechnology; clinical engineering; rehabilitation engineering
● Biochemical engineering and applications: metabolic pathway engineering; modeling and simulation
● Translational bioengineering
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