Ergothioneine Modulates Inflammatory Signaling in LPS-Stimulated RAW 264.7 Macrophages.

IF 3 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Cell Biochemistry and Biophysics Pub Date : 2026-09-25 DOI:10.1007/s12013-026-02179-w

Neil Patrick Uy, Hyeonjun Yu, Chang-Dae Lee, Soon Yeong Park, Hoon Kim, Sanghyun Lee

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引用次数: 0

Abstract

Ergothioneine (EGT), a naturally occurring antioxidant abundant in Hericium erinaceus, has been implicated in inflammation-related disorders, including inflammatory bowel disease (IBD). A validated HPLC-DAD method was developed for EGT quantification in H. erinaceus, demonstrating satisfactory specificity, linearity, precision, and accuracy. Network pharmacology analysis identified shared targets between EGT-associated proteins and IBD-related genes, with HSP90AA1 emerging as a key candidate. Molecular docking revealed stable binding of EGT within the HSP90AA1 active site. In lipopolysaccharide-stimulated RAW 264.7 macrophages, EGT significantly reduced the expression of pro-inflammatory mediators, including inducible nitric oxide synthase and cyclooxygenase-2, while modulating PPARγ expression. These findings provide integrated chemical and mechanistic evidence supporting EGT as a bioactive constituent of H. erinaceus with potential relevance for modulating intestinal inflammation. However, further studies are required to validate its therapeutic relevance in disease-specific models.

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麦角硫因调节lps刺激的RAW 264.7巨噬细胞的炎症信号。

麦角硫因（EGT）是猴头菌中丰富的天然抗氧化剂，与炎症相关疾病，包括炎症性肠病（IBD）有关。建立了一种有效的HPLC-DAD定量方法，具有良好的特异性、线性度、精密度和准确度。网络药理学分析发现了egt相关蛋白和ibd相关基因之间的共同靶点，其中HSP90AA1是一个关键的候选者。分子对接显示EGT在HSP90AA1活性位点稳定结合。在脂多糖刺激的RAW 264.7巨噬细胞中，EGT显著降低促炎介质的表达，包括诱导型一氧化氮合酶和环氧合酶-2，同时调节PPARγ的表达。这些发现提供了综合的化学和机制证据，支持EGT作为羊角杆菌的生物活性成分，与调节肠道炎症有潜在的相关性。然而，需要进一步的研究来验证其在疾病特异性模型中的治疗相关性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cell Biochemistry and Biophysics 生物-生化与分子生物学

CiteScore

4.40

自引率

0.00%

发文量

审稿时长

7.5 months

期刊介绍： Cell Biochemistry and Biophysics (CBB) aims to publish papers on the nature of the biochemical and biophysical mechanisms underlying the structure, control and function of cellular systems The reports should be within the framework of modern biochemistry and chemistry, biophysics and cell physiology, physics and engineering, molecular and structural biology. The relationship between molecular structure and function under investigation is emphasized. Examples of subject areas that CBB publishes are: · biochemical and biophysical aspects of cell structure and function; · interactions of cells and their molecular/macromolecular constituents; · innovative developments in genetic and biomolecular engineering; · computer-based analysis of tissues, cells, cell networks, organelles, and molecular/macromolecular assemblies; · photometric, spectroscopic, microscopic, mechanical, and electrical methodologies/techniques in analytical cytology, cytometry and innovative instrument design For articles that focus on computational aspects, authors should be clear about which docking and molecular dynamics algorithms or software packages are being used as well as details on the system parameterization, simulations conditions etc. In addition, docking calculations (virtual screening, QSAR, etc.) should be validated either by experimental studies or one or more reliable theoretical cross-validation methods.