{"title":"Oxibendazole induces cell cycle arrest and apoptosis in canine large-cell alimentary lymphoma cells.","authors":"Kosei Sakai, Masaki Hirao, Nana Suzuki, Shunsuke Shimamura","doi":"10.1292/jvms.26-0360","DOIUrl":null,"url":null,"abstract":"<p><p>Canine large-cell alimentary lymphoma (LCAL) responds poorly to chemotherapy and carries a poor prognosis. We investigated the antitumor effects of oxibendazole (OBZ), a benzimidazole anthelmintic identified by drug screening, in three canine LCAL cell lines. OBZ reduced cell viability in a dose-dependent manner, with half-maximal inhibitory concentration values of 0.08-0.16 μM. Cell cycle analysis showed increased G2/M and sub-G1 fractions, whereas an annexin V/propidium iodide assay demonstrated increased early and late apoptotic cells. Western blotting demonstrated caspase-3 cleavage following OBZ treatment. These findings indicate that OBZ exerts antitumor effects against canine LCAL cells by inducing G2/M cell cycle arrest and caspase-dependent apoptosis and suggest that OBZ may be a candidate for drug repurposing in canine LCAL.</p>","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":""},"PeriodicalIF":1.1000,"publicationDate":"2026-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Veterinary Medical Science","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1292/jvms.26-0360","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
Canine large-cell alimentary lymphoma (LCAL) responds poorly to chemotherapy and carries a poor prognosis. We investigated the antitumor effects of oxibendazole (OBZ), a benzimidazole anthelmintic identified by drug screening, in three canine LCAL cell lines. OBZ reduced cell viability in a dose-dependent manner, with half-maximal inhibitory concentration values of 0.08-0.16 μM. Cell cycle analysis showed increased G2/M and sub-G1 fractions, whereas an annexin V/propidium iodide assay demonstrated increased early and late apoptotic cells. Western blotting demonstrated caspase-3 cleavage following OBZ treatment. These findings indicate that OBZ exerts antitumor effects against canine LCAL cells by inducing G2/M cell cycle arrest and caspase-dependent apoptosis and suggest that OBZ may be a candidate for drug repurposing in canine LCAL.
期刊介绍：
JVMS is a peer-reviewed journal and publishes a variety of papers on veterinary science from basic research to applied science and clinical research. JVMS is published monthly and consists of twelve issues per year. Papers are from the areas of anatomy, physiology, pharmacology, toxicology, pathology, immunology, microbiology, virology, parasitology, internal medicine, surgery, clinical pathology, theriogenology, avian disease, public health, ethology, and laboratory animal science. Although JVMS has played a role in publishing the scientific achievements of Japanese researchers and clinicians for many years, it now also accepts papers submitted from all over the world.
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