{"title":"LAMP: Large-Area Molecular Mapping Platform","authors":"Zhiliang Huang, Wei Ju, Shuai Yan, Yage Chen, Shuai Wang, Yiran Li, Yue Huang, Na Li, Haozheng Li, Guang Yang, Sicheng Li, Xiangliang Yang, Yuan Tian, Huaqiu Zhang, Ping Wang","doi":"10.1021/acsphotonics.6c01459","DOIUrl":null,"url":null,"abstract":"<p>By providing unique chemical specificity and quantitative\r\nmetabolic\r\nimaging, label-free stimulated Raman scattering (SRS) and coherent\r\nanti-Stokes Raman scattering (CARS) microscopy is now emerging as\r\nthe next-generation clinical decision maker by providing molecular\r\ncontrast of cancerous tissues. However, the field of view (FOV) of\r\neither SRS or CARS is limited to hundreds of microns so far, due to\r\ntheir nonlinear nature demanding a tight focal point and instantaneous\r\nhigh peak energy. Here, we adopt a 1 MHz femtosecond pulse laser to\r\nboost the CARS signal and highly sensitive fly-eye SiPM detectors\r\nfor detection, and develop a large-area molecular mapping platform\r\n(LAMP) with an FOV of 3.5 × 3.5 cm<sup>2</sup>. LAMP combines\r\nCARS, second-harmonic generation (SHG), and two-photon excited fluorescence\r\n(TPEF), and achieves label-free multimodal imaging of intact bulk\r\nsurgical tissue specimens. We performed large-area multimodal imaging\r\nof ovarian cancer tissues and obtained structural distributions of\r\nlipid accumulation, collagen fibers, and natural pigments that exhibit\r\nautofluorescence. LAMP holds great promise for real clinical applications,\r\nincluding chemical pathology and surgical guidance.</p>","PeriodicalId":23,"journal":{"name":"ACS Photonics","volume":"13 19","pages":"5741–5748"},"PeriodicalIF":6.0000,"publicationDate":"2026-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS Photonics","FirstCategoryId":"101","ListUrlMain":"https://pubs.acs.org/doi/10.1021/acsphotonics.6c01459","RegionNum":1,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2026/9/22 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"MATERIALS SCIENCE, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 0
Abstract
By providing unique chemical specificity and quantitative
metabolic
imaging, label-free stimulated Raman scattering (SRS) and coherent
anti-Stokes Raman scattering (CARS) microscopy is now emerging as
the next-generation clinical decision maker by providing molecular
contrast of cancerous tissues. However, the field of view (FOV) of
either SRS or CARS is limited to hundreds of microns so far, due to
their nonlinear nature demanding a tight focal point and instantaneous
high peak energy. Here, we adopt a 1 MHz femtosecond pulse laser to
boost the CARS signal and highly sensitive fly-eye SiPM detectors
for detection, and develop a large-area molecular mapping platform
(LAMP) with an FOV of 3.5 × 3.5 cm2. LAMP combines
CARS, second-harmonic generation (SHG), and two-photon excited fluorescence
(TPEF), and achieves label-free multimodal imaging of intact bulk
surgical tissue specimens. We performed large-area multimodal imaging
of ovarian cancer tissues and obtained structural distributions of
lipid accumulation, collagen fibers, and natural pigments that exhibit
autofluorescence. LAMP holds great promise for real clinical applications,
including chemical pathology and surgical guidance.
期刊介绍：
Published as soon as accepted and summarized in monthly issues, ACS Photonics will publish Research Articles, Letters, Perspectives, and Reviews, to encompass the full scope of published research in this field.
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