Photobiomodulation Triggers M1/M2 Macrophage Polarization in Zymosan-Induced Acute Arthritis in Mice

IF 2 3区物理与天体物理 Q3 BIOCHEMICAL RESEARCH METHODS

Journal of Biophotonics Pub Date : 2026-09-21 DOI:10.1002/jbio.70366

Victória Batista Ferreira, Álvaro Carneiro de Souza, Lúcia Mara Januário dos Anjos, Ana Caroline da Silva Ferreira, Marcelle Abreu da Silva, Adenilson de Souza da Fonseca, Flávia de Paoli

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引用次数: 0

Abstract

Objective

To investigate whether photobiomodulation (PBM) modulates macrophage polarization in zymosan-induced acute joint inflammation, promoting an M1 to M2 phenotype shift.

Methods

Mice received intra-articular zymosan and PBM (830 nm, 10 mW) at 3 and 30 J/cm². Euthanasia occurred at 24, 48, and 72 h. Polarization was assessed via mRNA levels and protein expression.

Results

At 24 h, both fluences downregulated iNOS and Arg-1 mRNA. The 30 J/cm² dose sustained steady-state mRNA levels through 72 h. Immunohistochemistry showed that 3 J/cm² reduced M1 cells at 24 h, while 30 J/cm² was more effective in promoting M2 polarization at 48 and 72 h. Predominance of M2 over M1 macrophages was observed in all treated groups.

Conclusion

PBM may modulate acute joint inflammation by driving a phenotypic switch from M1 to M2 macrophages. These regulatory effects depend on irradiation conditions, with higher fluence (30 J/cm²) sustaining a durable pro-resolutive cellular profile.

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光生物调节在zymosana诱导的小鼠急性关节炎中触发M1/M2巨噬细胞极化。

目的：探讨光生物调节（PBM）是否在酶酶酶诱导的急性关节炎症中调节巨噬细胞极化，促进M1向M2表型转移。方法：小鼠关节内注射3和30 J/cm2的酶解糖和PBM （830 nm, 10 mW）。安乐死分别发生在24、48和72小时。通过mRNA水平和蛋白表达来评估极化。结果：24 h时，两者均影响下调的iNOS和Arg-1 mRNA。30 J/cm2剂量使mRNA水平持续72小时。免疫组化结果显示，3 J/cm2在24 h时使M1细胞减少，而30 J/cm2在48和72 h时更有效地促进M2极化。所有治疗组均观察到M2强于M1的巨噬细胞。结论：PBM可能通过驱动巨噬细胞M1向M2表型转换来调节急性关节炎症。这些调节作用取决于辐照条件，较高的辐照量（30焦耳/平方厘米）可维持持久的前分辨细胞轮廓。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Biophotonics 生物-生化研究方法

CiteScore

5.70

自引率

7.10%

发文量

248

审稿时长

1 months

期刊介绍： The first international journal dedicated to publishing reviews and original articles from this exciting field, the Journal of Biophotonics covers the broad range of research on interactions between light and biological material. The journal offers a platform where the physicist communicates with the biologist and where the clinical practitioner learns about the latest tools for the diagnosis of diseases. As such, the journal is highly interdisciplinary, publishing cutting edge research in the fields of life sciences, medicine, physics, chemistry, and engineering. The coverage extends from fundamental research to specific developments, while also including the latest applications.