Molecular characterization of a novel polymycovirus isolated from the phytopathogenic fungus Fusarium oxysporum

IF 2.5 4区医学 Q3 VIROLOGY

Archives of Virology Pub Date : 2026-09-21 DOI:10.1007/s00705-026-06739-6

Xiao Wu, Rui Guo, Ying Wang, Ze Lan Qiu, Xiang Ping Zhou, Jie Zhong, Yan Song Xiao, Tian Bo Liu

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引用次数: 0

Abstract

Here, a novel double-stranded RNA (dsRNA) mycovirus, tentatively designated Fusarium oxysporum polymycovirus 1 (FoPmV1), was isolated from Fusarium oxysporum, a phytopathogenic fungus that causes tobacco root rot. The genome of FoPmV1 consists of eight dsRNA segments, referred to as dsRNA1 to dsRNA8, with lengths of 2440 bp, 2276 bp, 2042 bp, 1303 bp, 1144 bp, 1064 bp, 1004 bp, and 913 bp, respectively. Sequence analysis revealed that these dsRNA segments encode 8 open reading frames (ORFs): ORF1 (771 amino acids, aa), ORF2 (699 aa), ORF3 (622 aa), and ORF4 (304 aa) encode an RNA-dependent RNA polymerase (RdRp), scaffold protein, methyltransferase, and coat protein (PASRP), respectively. While ORF5 (304 aa), ORF6 (161 aa), ORF7 (167 aa), and ORF8 (211 aa) encode proteins with unknown functions. BLAST analysis revealed that the RdRp of FoPmV1 shares the maximal sequence identity of 75.75% with the RdRp of Fusarium redolens polymycovirus 1. Phylogenetic analysis based on the RdRp sequence clustered FoPmV1 robustly within the family Polymycoviridae. Overall, this research represents the first report of a polymycovirus infecting F. oxysporum.

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从植物病原真菌尖孢镰刀菌中分离的一种新型多分枝病毒的分子特征。

本文从烟草根茎腐病真菌尖孢镰刀菌中分离到一种新型双链RNA （dsRNA）分枝病毒，暂命名为Fusarium oxysporum polymycovirus 1 （FoPmV1）。FoPmV1基因组包含8个dsRNA片段，分别为2440 bp、2276 bp、2042 bp、1303 bp、1144 bp、1064 bp、1004 bp和913 bp，分别为dsRNA1 ~ dsRNA8。序列分析显示，这些dsRNA片段编码8个开放阅读框（orf）： ORF1（771个氨基酸，aa）、ORF2（699个氨基酸）、ORF3（622个氨基酸）和ORF4（304个氨基酸）分别编码RNA依赖性RNA聚合酶（RdRp）、支架蛋白、甲基转移酶和外壳蛋白（PASRP）。ORF5 （304 aa）、ORF6 （161 aa）、ORF7 （167 aa）和ORF8 （211 aa）编码功能未知的蛋白。BLAST分析结果显示，FoPmV1的RdRp与红镰刀菌多分枝病毒1的RdRp最大序列同源性为75.75%。基于RdRp序列的系统发育分析将FoPmV1稳稳地聚集在多分枝病毒科。总的来说，本研究是首次报道多分枝病毒感染尖孢镰刀菌。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Archives of Virology 医学-病毒学

CiteScore

5.10

自引率

7.40%

发文量

324

审稿时长

4.5 months

期刊介绍： Archives of Virology publishes original contributions from all branches of research on viruses, virus-like agents, and virus infections of humans, animals, plants, insects, and bacteria. Coverage spans a broad spectrum of topics, from descriptions of newly discovered viruses, to studies of virus structure, composition, and genetics, to studies of virus interactions with host cells, organisms and populations. Studies employ molecular biologic, molecular genetics, and current immunologic and epidemiologic approaches. Contents include studies on the molecular pathogenesis, pathophysiology, and genetics of virus infections in individual hosts, and studies on the molecular epidemiology of virus infections in populations. Also included are studies involving applied research such as diagnostic technology development, monoclonal antibody panel development, vaccine development, and antiviral drug development.Archives of Virology wishes to publish obituaries of recently deceased well-known virologists and leading figures in virology.