D2O metabolic labeling combined with AuNPs@PVP/SA hydrogel SERS platform for rapid quantitative detection of active lactic acid bacteria

IF 5.4 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta Pub Date : 2026-09-21 DOI:10.1007/s00604-026-08404-w

Shijie Liu, Lijun Zhao, Yueyu Bai, Qian Ding, Yangyang Ma, Miaoyun Li, Dong Liang, Yanxia Liu, Haoyun Li, Lingxia Sun

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引用次数: 0

Abstract

Achieving accurate quantification and counting of active lactic acid bacteria (LAB) is an important technical guarantee for the industrial production and application of LAB and their products. In this study, a gold nanoparticles@polyvinylpyrrolidone/sodium alginate (AuNPs@PVP/SA) hydrogel SERS sensor with outstanding stability and sensitivity was developed. Utilizing this platform as a substrate material to mitigate the “coffee ring” effect, we explored a strategy for the precise quantitative determination of mixed active LAB based on the AuNPs@PVP/SA hydrogel SERS platform and D₂O-probed Raman spectroscopy technology. The results indicated that the SERS platform based on AuNPs@PVP/SA hydrogel exhibited excellent spectral reproducibility for LAB as a SERS substrate material, with a significantly reduced “coffee ring” effect. This strategy enabled rapid and quantitative detection of active lactic acid bacteria (LAB), with a limit of detection (LOD) of 10 CFU/mL. Moreover, the strategy had excellent analytical capabilities for the qualitative identification and precise quantification of different species of active LAB in actual samples, achieving an average accuracy of 98.67% with RSD values all less than 5%. It provides a potential technical support for the dynamic monitoring of relative abundance and metabolic phenomena of mixed active LAB in co-culture systems during actual industrial production.

Graphical Abstract

查看原文本刊更多论文

D2O代谢标记结合AuNPs@PVP/SA水凝胶SERS平台快速定量检测活性乳酸菌。

实现活性乳酸菌（LAB）的准确定量和计数是实现乳酸菌及其产品工业化生产和应用的重要技术保证。本研究开发了一种稳定性和灵敏度优异的金nanoparticles@polyvinylpyrrolidone/海藻酸钠（AuNPs@PVP/SA）水凝胶SERS传感器。利用该平台作为衬底材料，缓解“咖啡环”效应，我们探索了一种基于AuNPs@PVP/SA水凝胶SERS平台和d20探针拉曼光谱技术的混合活性LAB精确定量测定策略。结果表明，基于AuNPs@PVP/SA水凝胶的SERS平台对LAB作为SERS底物具有良好的光谱再现性，显著降低了“咖啡环”效应。该策略能够快速定量检测活性乳酸菌（LAB），检出限（LOD）为10 CFU/mL。该策略对实际样品中不同种类的活性LAB具有良好的定性鉴定和精确定量分析能力，平均准确度为98.67%，RSD值均小于5%。为实际工业生产中混养系统中混合活性LAB的相对丰度和代谢现象的动态监测提供了潜在的技术支持。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Microchimica Acta 化学-分析化学

CiteScore

9.80

自引率

5.30%

发文量

410

审稿时长

2.7 months

期刊介绍： As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.