Intraday and Interday Repeatability of Laser Speckle Contrast Imaging for Ocular Blood Flow Measurement in Healthy Subjects.

IF 2.7 3区医学 Q2 OPHTHALMOLOGY

Translational Vision Science & Technology Pub Date : 2026-09-01 DOI:10.1167/tvst.15.9.12

Xiaogang Wang, Giovana Rosa Gameiro, Andrew Hoover, Mariam Moarefi, Shuya Wang, Lian Duan, Xiaoming Wang, Hong Jiang, Jianhua Wang

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引用次数: 0

Abstract

Purpose: To investigate the intra- and interday repeatability of ocular blood flow velocity index (BFVi) measurements using a laser speckle contrast imaging device.

Methods: BFVi measurements were obtained using XyCAM CRE (Vasoptic Medical Inc., Columbia, MD). Two imaging sessions were performed on the same day (26 eyes) and on different days (20 eyes). For optic nerve head-centered datasets, the analyzed regions included the overall field of view, the optic disc, and a standard temporal peripapillary region. For macula-centered datasets, the regions analyzed were the overall field of view, the foveal avascular zone, and the perifoveal region. For each region, heart rate-adjusted measurements of the peak, dip, and mean BFVi were assessed.

Results: For intraday measurements of all the optic nerve head-centered parameters, the coefficients of variation (CVs) ranged from 4.8% to 8.5%, and the intraclass correlation coefficient ranged from 0.74 to 0.98. For all macula-centered parameters, the CVs ranged from 4.7% to 6.2%, and the ICCs ranged from 0.93 to 0.98. For the interday measurements of all the optic nerve head-centered parameters, the CVs ranged from 7.9% to 12.6%, and the ICCs ranged from 0.56 to 0.93. For all the macula-centered parameters, the CVs ranged from 8.6% to 15.0%, and the intraclass correlation coefficient ranged from 0.79 to 0.94.

Conclusions: Laser speckle contrast imaging demonstrated strong intraday repeatability and reliability for BFVi measurement in healthy subjects, with good interday reliability for key parameters despite some variability.

Translational relevance: Laser speckle contrast imaging shows promise for clinical use in ophthalmology due to its robust intraday and good interday reliability for ocular blood flow velocity measurements.

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健康受试者眼血流测量激光散斑对比成像的日间和日间重复性。

目的：探讨激光散斑对比成像仪测量眼血流速度指数（BFVi）的日、日间重复性。方法：使用XyCAM CRE （Vasoptic Medical Inc., Columbia， MD）测量BFVi。在同一天（26只眼）和不同的日子（20只眼）进行两次成像。对于视神经头为中心的数据集，分析的区域包括整个视野、视盘和标准的颞部乳头周围区域。对于以黄斑为中心的数据集，分析的区域是整个视野、中央凹无血管区和中央凹周围区。对于每个区域，评估心率调整后的峰值、下降和平均BFVi测量值。结果：视神经头中心参数的变异系数（cv）为4.8% ~ 8.5%，类内相关系数为0.74 ~ 0.98。对于所有以黄斑为中心的参数，cv范围为4.7% ~ 6.2%，ICCs范围为0.93 ~ 0.98。在日间测量视神经头为中心的所有参数时，cv范围为7.9% ~ 12.6%，ICCs范围为0.56 ~ 0.93。所有以黄斑为中心的参数的cv值为8.6% ~ 15.0%，类内相关系数为0.79 ~ 0.94。结论：激光散斑对比成像对健康受试者的BFVi测量具有较强的日间重复性和可靠性，关键参数的日间可靠性较好，但存在一定的变异性。翻译相关性：激光散斑对比成像由于其强大的日间和日间眼部血流速度测量的可靠性，在眼科临床应用中显示出良好的前景。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Translational Vision Science & Technology Engineering-Biomedical Engineering

CiteScore

5.70

自引率

3.30%

发文量

346

审稿时长

25 weeks

期刊介绍： Translational Vision Science & Technology (TVST), an official journal of the Association for Research in Vision and Ophthalmology (ARVO), an international organization whose purpose is to advance research worldwide into understanding the visual system and preventing, treating and curing its disorders, is an online, open access, peer-reviewed journal emphasizing multidisciplinary research that bridges the gap between basic research and clinical care. A highly qualified and diverse group of Associate Editors and Editorial Board Members is led by Editor-in-Chief Marco Zarbin, MD, PhD, FARVO. The journal covers a broad spectrum of work, including but not limited to: Applications of stem cell technology for regenerative medicine, Development of new animal models of human diseases, Tissue bioengineering, Chemical engineering to improve virus-based gene delivery, Nanotechnology for drug delivery, Design and synthesis of artificial extracellular matrices, Development of a true microsurgical operating environment, Refining data analysis algorithms to improve in vivo imaging technology, Results of Phase 1 clinical trials, Reverse translational ("bedside to bench") research. TVST seeks manuscripts from scientists and clinicians with diverse backgrounds ranging from basic chemistry to ophthalmic surgery that will advance or change the way we understand and/or treat vision-threatening diseases. TVST encourages the use of color, multimedia, hyperlinks, program code and other digital enhancements.