Antibacterial and wound-healing potential of Garcinia mangostana leaves against Staphylococcus epidermidis - infected mice

IF 4.6 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology Pub Date : 2026-09-01 Epub Date: 2026-09-04 DOI:10.1016/j.bcab.2026.104231

Krishnan Raguvaran, Marya Salfia Khoerunnisah, Siswadi, Abdi Wira Septama, Andhika Yudha Prawira, Siska Kusumastuti, Rizna Triana Dewi, Ahmad Randy, Tia Okselni, Marissa Angelina

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Abstract

This study investigates the antibacterial and wound-healing potential of Garcinia mangostana leaf ethyl acetate extract (GMLEA) against Staphylococcus epidermidis. GMLEA exhibited strong antibacterial activity, producing an inhibition zone of 16.0 mm at 1 mg/mL, with minimum inhibitory and bactericidal concentrations (MIC/MBC) of 125 μg/mL and 500 μg/mL, respectively. Biofilm assays revealed up to 88.34% inhibition and concentration-dependent reduction of extracellular polymeric substances (EPS), while FT-IR analysis indicated structural changes in EPS associated with biofilm integrity. Membrane integrity assays showed intracellular protein and DNA leakage, confirming bacteriolytic effects. GMLEA also demonstrated notable antioxidant activity, with 88.38% (DPPH) and 90.12% (FRAP) scavenging at 200 μg/mL. In S. epidermidis-infected mice, GMLEA accelerated wound closure, reduced inflammation, and promoted epithelial and hair follicle regeneration, achieving complete healing by day 15 at 500 μg/mL. Toxicity tests confirmed low cytotoxicity in Vero cells and no adverse effects on liver or kidney biomarkers. HR-LCMS analysis identified xanthones as the major bioactive compounds. These findings highlight GMLEA as a promising natural antimicrobial and wound-healing agent.

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山竹藤叶对表皮葡萄球菌感染小鼠的抗菌和创面愈合潜力

研究了山竹藤叶乙酸乙酯提取物（GMLEA）对表皮葡萄球菌的抑菌和创面愈合作用。GMLEA表现出较强的抑菌活性，在1 mg/mL时产生16.0 mm的抑制带，最小抑菌浓度（MIC/MBC）分别为125 μg/mL和500 μg/mL。生物膜分析显示，细胞外聚合物（EPS）的抑制率高达88.34%，并呈浓度依赖性降低，而FT-IR分析表明，EPS的结构变化与生物膜的完整性有关。膜完整性检测显示细胞内蛋白和DNA渗漏，证实其有溶菌作用。GMLEA具有显著的抗氧化活性，在200 μg/mL时，DPPH和FRAP的清除率分别为88.38%和90.12%。在表皮葡萄球菌感染的小鼠中，GMLEA加速伤口愈合，减少炎症，促进上皮和毛囊再生，在500 μg/mL的浓度下在第15天完全愈合。毒性试验证实Vero细胞毒性低，对肝脏或肾脏生物标志物无不良影响。HR-LCMS分析鉴定其主要活性成分为山酮类。这些发现突出了GMLEA作为一种有前途的天然抗菌和伤口愈合剂。

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来源期刊

Biocatalysis and agricultural biotechnology Agricultural and Biological Sciences-Agronomy and Crop Science

CiteScore

7.70

自引率

2.50%

发文量

308

审稿时长

48 days

期刊介绍： Biocatalysis and Agricultural Biotechnology is the official journal of the International Society of Biocatalysis and Agricultural Biotechnology (ISBAB). The journal publishes high quality articles especially in the science and technology of biocatalysis, bioprocesses, agricultural biotechnology, biomedical biotechnology, and, if appropriate, from other related areas of biotechnology. The journal will publish peer-reviewed basic and applied research papers, authoritative reviews, and feature articles. The scope of the journal encompasses the research, industrial, and commercial aspects of biotechnology, including the areas of: biocatalysis; bioprocesses; food and agriculture; genetic engineering; molecular biology; healthcare and pharmaceuticals; biofuels; genomics; nanotechnology; environment and biodiversity; and bioremediation.