Freeze-thaw-directed interfacial assembly of a gold nanoparticle/MXene electrochemical aptamer sensor for antibody-free myeloperoxidase detection

IF 5.4 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta Pub Date : 2026-09-18 DOI:10.1007/s00604-026-08406-8

Congyu Min, Haifeng Sun, Haixia Zhang, Jinzhu Zhang, Min Ding, Xiaoqing Zhang

{"title":"Freeze-thaw-directed interfacial assembly of a gold nanoparticle/MXene electrochemical aptamer sensor for antibody-free myeloperoxidase detection","authors":"Congyu Min, Haifeng Sun, Haixia Zhang, Jinzhu Zhang, Min Ding, Xiaoqing Zhang","doi":"10.1007/s00604-026-08406-8","DOIUrl":null,"url":null,"abstract":"<div><p>An electrochemical aptamer-based (E-AB) sensor for myeloperoxidase (MPO) was developed that combined a gold nanoparticle /MXene scaffold with freeze-thaw-directed aptamer immobilization and probe-density optimization. Unlike using nanocomposite amplification alone, this strategy regulates probe density and accessibility at the bioreceptor layer to improve target-coupled signal transduction. Using the same aptamer solution, the freeze-thaw method yielded 60.77% greater MPO-induced signal suppression and a 2.4-fold lower regression-derived limit of detection (LOD) than conventional incubation. After target incubation for 50 min at 4 °C, the sensor showed a linear response from 5.00 to 250.0 ng/mL in 1% plasma matrix (equivalent to 500 ~ 25,000 ng/mL in undiluted plasma), with an LOD of 4.15 ng/mL (equivalent to 415 ng/mL in undiluted plasma). These results illustrated that coupling conductive nanointerface design with aptamer-layer organization might provide a practical route for antibody-free and H<sub>2</sub>O<sub>2</sub>-independent MPO determination.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"193 10","pages":""},"PeriodicalIF":5.4000,"publicationDate":"2026-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microchimica Acta","FirstCategoryId":"92","ListUrlMain":"https://link.springer.com/article/10.1007/s00604-026-08406-8","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}

引用次数: 0

Abstract

An electrochemical aptamer-based (E-AB) sensor for myeloperoxidase (MPO) was developed that combined a gold nanoparticle /MXene scaffold with freeze-thaw-directed aptamer immobilization and probe-density optimization. Unlike using nanocomposite amplification alone, this strategy regulates probe density and accessibility at the bioreceptor layer to improve target-coupled signal transduction. Using the same aptamer solution, the freeze-thaw method yielded 60.77% greater MPO-induced signal suppression and a 2.4-fold lower regression-derived limit of detection (LOD) than conventional incubation. After target incubation for 50 min at 4 °C, the sensor showed a linear response from 5.00 to 250.0 ng/mL in 1% plasma matrix (equivalent to 500 ~ 25,000 ng/mL in undiluted plasma), with an LOD of 4.15 ng/mL (equivalent to 415 ng/mL in undiluted plasma). These results illustrated that coupling conductive nanointerface design with aptamer-layer organization might provide a practical route for antibody-free and H₂O₂-independent MPO determination.

Graphical abstract

查看原文本刊更多论文

用于无抗体髓过氧化物酶检测的金纳米颗粒/MXene电化学适体传感器的冻融定向界面组装

开发了一种基于电化学适配体（E-AB）的髓过氧化物酶（MPO）传感器，该传感器将金纳米颗粒/MXene支架与冻融定向适配体固定和探针密度优化相结合。与单独使用纳米复合材料扩增不同，该策略调节探针密度和生物受体层的可达性，以改善靶标耦合信号转导。使用相同的适体溶液，冻融法比常规孵育法对mpo诱导的信号抑制效果高60.77%，回归衍生检测限（LOD）低2.4倍。在4℃下靶培养50分钟后，传感器在1%血浆基质（相当于未稀释血浆中的500 ~ 25,000 ng/mL）中显示出5.00 ~ 250.0 ng/mL的线性响应，LOD为4.15 ng/mL（相当于未稀释血浆中的415 ng/mL）。这些结果表明，耦合导电纳米界面设计与适体层组织可能为无抗体和不依赖h2o2的MPO测定提供了一条实用的途径。图形抽象

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Microchimica Acta 化学-分析化学

CiteScore

9.80

自引率

5.30%

发文量

410

审稿时长

2.7 months

期刊介绍： As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.