Ultrabright AIE-MOF-based Lateral Flow Immunoassay for Rapid Detection of Burkholderia gladioli

IF 3 3区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Food Analytical Methods Pub Date : 2026-09-15 DOI:10.1007/s12161-026-03276-5

Huiya Wei, Jinbo Cao, Lu Han, Xianglong Xu, Qixuan Jin, Xiaoya Li, Hui Yu, Xiujin Chen, Zhaozhou Li, Yao Wang, Hui Chen

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引用次数: 0

Abstract

Burkholderia gladioli (B. gladioli), a foodborne pathogen, poses a significant threat to global food safety because of its adverse effects on human health. Conventional detection methods are often labor-intensive, time-consuming, and costly, highlighting the urgent need for rapid, simple, and sensitive detection strategies. Lateral flow immunoassay (LFIA) has been widely adopted for point-of-care testing (POCT) owing to its simplicity, portability, and rapid response. In this study, an aggregation-induced emission metal–organic framework (AIE-MOF)-based LFIA (AIE-MOF-LFIA) was developed for the rapid on-site detection of B. gladioli. The proposed assay achieved a detection limit of 72 CFU/mL, representing an approximately 25-fold improvement in sensitivity over conventional gold-nanoparticle-based LFIA (AuNP-LFIA). No cross-reactivity was observed with eight common foodborne pathogens, and both intra- and inter-batch coefficients of variation (CVs) remained below 10%. Recoveries from wood ears, yellow corn flour, and wet rice noodles samples ranged from 83.92% to 113.75%. Moreover, distinct fluorescent signals were generated within 10 min, enabling rapid visual detection. The proposed assay features simple operation, supporting both naked-eye qualitative analysis and instrument-assisted quantitative detection, together with good analytical performance. It provides a practical platform for rapid on-site screening of B. gladioli in high-risk foods and facilitates early food safety risk assessment and intervention.

Graphical Abstract

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基于ae - mof的超亮横向流动免疫分析法快速检测剑兰伯克氏菌

剑兰伯克霍尔德菌（B. gladioli）是一种食源性病原体，对人类健康产生不利影响，对全球食品安全构成重大威胁。传统的检测方法往往是劳动密集型、耗时和昂贵的，因此迫切需要快速、简单和敏感的检测策略。侧流免疫分析法（LFIA）因其简单、便携和快速反应而被广泛应用于即时检测（POCT）。本研究建立了一种基于聚集诱导发射金属有机框架（AIE-MOF）的LFIA (AIE-MOF-LFIA)，用于剑兰白花的快速现场检测。该方法的检测限为72 CFU/mL，比传统的基于金纳米颗粒的LFIA （AuNP-LFIA）的灵敏度提高了约25倍。8种常见食源性致病菌无交叉反应，批内和批间变异系数（cv）均在10%以下。木穗、黄玉米粉和湿米粉样品的回收率为83.92% ~ 113.75%。此外，在10分钟内产生明显的荧光信号，实现了快速的视觉检测。该方法操作简单，既支持裸眼定性分析，也支持仪器辅助定量检测，分析性能良好。为高危险食品中剑兰杆菌的现场快速筛查提供了实用平台，便于早期食品安全风险评估和干预。图形抽象

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来源期刊

Food Analytical Methods 农林科学-食品科技

CiteScore

6.00

自引率

3.40%

发文量

244

审稿时长

3.1 months

期刊介绍： Food Analytical Methods publishes original articles, review articles, and notes on novel and/or state-of-the-art analytical methods or issues to be solved, as well as significant improvements or interesting applications to existing methods. These include analytical technology and methodology for food microbial contaminants, food chemistry and toxicology, food quality, food authenticity and food traceability. The journal covers fundamental and specific aspects of the development, optimization, and practical implementation in routine laboratories, and validation of food analytical methods for the monitoring of food safety and quality.