Development of starch–alginate–PVA hydrogel beads for encapsulation and sustained delivery of Trichoderma longibrachiatum in Capsicum annuum

IF 4.2 3区化学 Q2 POLYMER SCIENCE

Polymer Bulletin Pub Date : 2026-09-15 DOI:10.1007/s00289-026-06690-1

Bandana Padhan, Joyjyoti Das, Parswati Sen, Kartik Charan Lenka

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引用次数: 0

Abstract

Sustainable microbial delivery systems can improve the stability and effectiveness of bioinoculants in agriculture. In this study, a starch–alginate–PVA hydrogel was developed to encapsulate Trichoderma longibrachiatum by ionic gelation. The beads showed uniform spherical morphology, high moisture retention (86.17 ± 1.8%), swelling capacity (233 ± 5.4%), and encapsulation efficiency (87.0 ± 2.0%). Scanning electron microscopy revealed a porous, interconnected structure, while FTIR and XRD confirmed polymer interactions and a predominantly amorphous matrix. The encapsulated formulation maintained 8.0 × 10⁷ CFU g⁻¹ bead after 45 days of storage and provided sustained availability of viable propagules in soil. Its plant growth-promoting performance was evaluated in Capsicum annuum under pot conditions and compared with Trichoderma slurry and untreated controls. At 30 days, encapsulated treatment increased plant height, leaf number, leaf area, and dry biomass to 72.3 ± 3.0 cm, 98.4 ± 3.4, 56.8 ± 2.7 cm², and 18.9 ± 0.5 g, respectively. It also improved chlorophyll and carotenoid contents and enhanced antioxidant defense. At 10 days, SOD, APX, GPX, and CAT activities increased by 36.4%, 73.0%, 72.2%, and 54.9%, respectively, relative to the untreated control, while APX showed a 149.5% increase at 30 days. Overall, the hydrogel supported fungal survival and sustained delivery while promoting plant growth and reducing oxidative stress, demonstrating its potential as an eco-friendly delivery platform for Trichoderma-based bioinoculants in sustainable agriculture.

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淀粉-海藻酸盐-聚乙烯醇水凝胶微球在辣椒中的包封和持续递送

可持续的微生物输送系统可以提高农业生物接种剂的稳定性和有效性。本研究制备了一种淀粉-海藻酸盐-聚乙烯醇水凝胶，通过离子凝胶对长achiachiatum木霉进行包封。微球形态均匀，具有较高的保湿率（86.17±1.8%）、溶胀率（233±5.4%）和包封率（87.0±2.0%）。扫描电子显微镜显示了多孔的、相互连接的结构，而FTIR和XRD证实了聚合物相互作用和主要的无定形基质。封装配方在储存45天后保持8.0 × 10⁷CFU g - 1毒珠，并在土壤中提供持续的可活繁殖体。在盆栽条件下，对其在辣椒中的促生性能进行了评价，并与木霉浆和未处理对照进行了比较。在30 d时，包封处理使株高、叶数、叶面积和干生物量分别增加到72.3±3.0 cm、98.4±3.4 cm、56.8±2.7 cm²和18.9±0.5 g。它还提高了叶绿素和类胡萝卜素的含量，增强了抗氧化防御。第10天，SOD、APX、GPX和CAT活性分别比未处理的对照提高36.4%、73.0%、72.2%和54.9%，而APX在第30天提高了149.5%。总体而言，水凝胶支持真菌存活和持续递送，同时促进植物生长和减少氧化应激，表明其作为可持续农业中基于木霉菌的生物接种剂的环保递送平台的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Polymer Bulletin 化学-高分子科学

CiteScore

6.00

自引率

6.20%

发文量

审稿时长

5.5 months

期刊介绍： "Polymer Bulletin" is a comprehensive academic journal on polymer science founded in 1988. It was founded under the initiative of the late Mr. Wang Baoren, a famous Chinese chemist and educator. This journal is co-sponsored by the Chinese Chemical Society, the Institute of Chemistry, and the Chinese Academy of Sciences and is supervised by the China Association for Science and Technology. It is a core journal and is publicly distributed at home and abroad. "Polymer Bulletin" is a monthly magazine with multiple columns, including a project application guide, outlook, review, research papers, highlight reviews, polymer education and teaching, information sharing, interviews, polymer science popularization, etc. The journal is included in the CSCD Chinese Science Citation Database. It serves as the source journal for Chinese scientific and technological paper statistics and the source journal of Peking University's "Overview of Chinese Core Journals."