{"title":"<i>Arabidopsis</i> F-BOX STRESS INDUCED (FBS) proteins have distinct subcellular interaction patterns with 14-3-3 proteins.","authors":"Mael Bradley, Meghan Bacher, Kaitlin Riggan, Nicolo Torresan, Megan Tegman, Sabine Angier, Amy Replogle, Bryan Thines","doi":"10.17912/micropub.biology.002221","DOIUrl":null,"url":null,"abstract":"<p><p>F-BOX STRESS INDUCED (FBS) proteins are substrate adaptors in SCF-type E3 ubiquitin ligases that regulate plant development and stress responses. <i>Arabidopsis thaliana</i> FBS1 (AT1G61340) interacts with multiple 14-3-3 proteins, but it is unknown whether other FBS family members share these interactions. Bimolecular fluorescence complementation (BiFC) assays in <i>Nicotiana benthamiana</i> showed that all four FBS proteins interact with multiple 14-3-3 proteins in vivo. However, FBS1 interactions were restricted to the nucleus, whereas FBS2 - FBS4 (AT4G21510, AT4G05010, AT4G35930) interactions were detected in both the nucleus and cytosol. These findings indicate conserved 14-3-3 interactions within the FBS family, but suggest functional divergence in subcellular interaction profiles.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2026 ","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2026-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13575791/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"microPublication biology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.17912/micropub.biology.002221","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2026/1/1 0:00:00","PubModel":"eCollection","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
F-BOX STRESS INDUCED (FBS) proteins are substrate adaptors in SCF-type E3 ubiquitin ligases that regulate plant development and stress responses. Arabidopsis thaliana FBS1 (AT1G61340) interacts with multiple 14-3-3 proteins, but it is unknown whether other FBS family members share these interactions. Bimolecular fluorescence complementation (BiFC) assays in Nicotiana benthamiana showed that all four FBS proteins interact with multiple 14-3-3 proteins in vivo. However, FBS1 interactions were restricted to the nucleus, whereas FBS2 - FBS4 (AT4G21510, AT4G05010, AT4G35930) interactions were detected in both the nucleus and cytosol. These findings indicate conserved 14-3-3 interactions within the FBS family, but suggest functional divergence in subcellular interaction profiles.
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