Deeptha Vasudevan, Yi-Wen Wang, Hannah Carr, Elise Paniel, Sean Corcoran, Chris C Wreden, Elaine Kushkowski, Conor Lee-Smith, Ellie S Heckscher
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引用次数: 0
Abstract
Neural stem cells are highly flexible, generating different neuronal lineages depending on context. In the Drosophila CNS, 30 types of neural stem cells, or neuroblasts, are repeated in each segment as serial homologs, but produce distinct circuits specialized for different body regions. The full in vivo heterogeneity in stem cell development is still poorly understood. This study aimed to uncover the cellular mechanisms behind the development of regionally specialized circuits from serially homologous neuroblasts, focusing on NB3-3 lineages as a model. Using lineage tracing, Notch manipulations, marker analysis, cell death blockade and circuit tracing, we mapped neurogenesis and circuit assembly for each NB3-3 lineage across all segments. Our results show that serially homologous neuroblasts in certain regions modify proliferation patterns, adding or omitting temporal cohorts, leading to extra neurons for specialized circuits. Four types of temporal cohorts are produced, but one type, which we term a 'fundamental' temporal cohort, appears in every region. Neurons from this cohort are further refined post-mitotically, enabling regional circuit specialization. Our study reveals a framework for the heterogeneous development of serially homologous stem cells.
期刊介绍：
Development’s scope covers all aspects of plant and animal development, including stem cell biology and regeneration. The single most important criterion for acceptance in Development is scientific excellence. Research papers (articles and reports) should therefore pose and test a significant hypothesis or address a significant question, and should provide novel perspectives that advance our understanding of development. We also encourage submission of papers that use computational methods or mathematical models to obtain significant new insights into developmental biology topics. Manuscripts that are descriptive in nature will be considered only when they lay important groundwork for a field and/or provide novel resources for understanding developmental processes of broad interest to the community.
Development includes a Techniques and Resources section for the publication of new methods, datasets, and other types of resources. Papers describing new techniques should include a proof-of-principle demonstration that the technique is valuable to the developmental biology community; they need not include in-depth follow-up analysis. The technique must be described in sufficient detail to be easily replicated by other investigators. Development will also consider protocol-type papers of exceptional interest to the community. We welcome submission of Resource papers, for example those reporting new databases, systems-level datasets, or genetic resources of major value to the developmental biology community. For all papers, the data or resource described must be made available to the community with minimal restrictions upon publication.
To aid navigability, Development has dedicated sections of the journal to stem cells & regeneration and to human development. The criteria for acceptance into these sections is identical to those outlined above. Authors and editors are encouraged to nominate appropriate manuscripts for inclusion in one of these sections.
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