Optimizing yeast surface display efficiency: a multifaceted strategy analysis.

IF 8.7 2区工程技术 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Critical Reviews in Biotechnology Pub Date : 2026-09-15 DOI:10.1080/07388551.2026.2728042

Fanyu Chen, Chaowei Zhou, Peifang Ren, Tianpeng Chen, Yu Sha, Wenjun Sun, Yong Chen

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引用次数: 0

Abstract

Microbial cell surface display systems represent a promising and robust approach for engineering whole-cell biocatalysts, with broad applications in biotechnology and biomedicine. This methodology overcomes limitations in substrate transport, eliminates the laborious and time-consuming process of enzyme purification, and enhances reusability and regenerability in repeated batch processes, thereby delivering economic benefits. Among various host systems, yeast surface display (YSD) offers unique advantages, such as large cell size, ease of culture and genetic manipulation, and the capacity for complete post-translational processing of secreted proteins. This review highlights strategies to improve the efficiency of YSD from novel perspectives, including intracellular molecular transcription, cell surface engineering, and external spatial expansion. Additionally, it comprehensively summarizes the practical applications of YSD in biocatalysis, underscoring their transformative potential in industrial and biomedical contexts.

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优化酵母表面显示效率：一个多方面的策略分析。

微生物细胞表面显示系统代表了一种很有前途和强大的全细胞生物催化剂工程方法，在生物技术和生物医学中有着广泛的应用。该方法克服了底物运输的限制，消除了酶纯化的费力和耗时的过程，并提高了重复批次过程中的可重用性和可再生性，从而带来了经济效益。在多种宿主系统中，酵母表面显示（YSD）具有细胞体积大、易于培养和遗传操作、能够对分泌蛋白进行完整的翻译后加工等独特优势。本文从细胞内分子转录、细胞表面工程和外部空间扩展等方面综述了提高细胞内分子转录效率的策略。此外，它全面总结了YSD在生物催化中的实际应用，强调了它们在工业和生物医学背景下的变革潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Critical Reviews in Biotechnology 工程技术-生物工程与应用微生物

CiteScore

20.80

自引率

1.10%

发文量

审稿时长

4.8 months

期刊介绍： Biotechnological techniques, from fermentation to genetic manipulation, have become increasingly relevant to the food and beverage, fuel production, chemical and pharmaceutical, and waste management industries. Consequently, academic as well as industrial institutions need to keep abreast of the concepts, data, and methodologies evolved by continuing research. This journal provides a forum of critical evaluation of recent and current publications and, periodically, for state-of-the-art reports from various geographic areas around the world. Contributing authors are recognized experts in their fields, and each article is reviewed by an objective expert to ensure accuracy and objectivity of the presentation.