Accessing Polyphenols Effect on Bond Strength and Dentin Enzymatic Degradation

IF 2.4 Q3 DENTISTRY, ORAL SURGERY & MEDICINE

Clinical and Experimental Dental Research Pub Date : 2026-09-15 DOI:10.1002/cre2.70449

Ana Catarina Rios Castro Alves, Adonias Antonio da Silva, Roberta Bruno da Silva, Rayan de Lima França, Dayse Andrade Romão, de Fragoso, Natanael Barbosa dos Santos, de Porto

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引用次数: 0

Abstract

Introduction

Polyphenols have been investigated in dental materials and restorative techniques due to their interactions with metal cations, antioxidant activity, and collagen crosslinking ability, which make them a promise for reinforcing the dental substrate and improving material adhesion. However, more studies are necessary before to support their use in clinical procedures.

Objectives

This study evaluated the effects of quercetin and resveratrol on bond strength and the protection of dentin collagen fibrils against enzymatic degradation.

Materials and Methods

Demineralized human dentin specimens were immersed for 1 min in solutions of quercetin or resveratrol (100, 250, and 500 µg/mL), 2% chlorhexidine (CHX), or 0.9% saline (control). The specimens were then incubated in collagenase for 24 h at 37°C, and changes in dry mass were measured. For microtensile bond strength testing, quercetin, resveratrol, CHX, or distilled water (DW) were applied to phosphoric acid–conditioned dentin, followed by application of Single Bond 2 (3 M/ESPE) adhesive and Z350XT resin (3 M/ESPE). Adhesive strength was measured after 24 h and after 1 year in distilled water. Data were analyzed using the Kruskal–Wallis and Dunn tests or ANOVA followed by Tukey's test (p < 0.05).

Results

Quercetin-treated specimens showed a significant weight increase compared with CHX (p < 0.05). Both quercetin- and resveratrol-treated groups exhibited resistance to collagenase similar to CHX (p > 0.05). After 1 year, only the DW group showed a significant decrease in bond strength (p = 0.001).

Conclusions

Dentin treatment with quercetin or resveratrol effectively preserved adhesive strength and protected collagen against enzymatic degradation, suggesting their potential as agents to improve the longevity of adhesive restorations.

查看原文本刊更多论文

接近多酚对牙本质酶降解和结合强度的影响。

导读：由于多酚与金属阳离子的相互作用、抗氧化活性和胶原交联能力，多酚在牙科材料和修复技术中得到了广泛的研究，这使得它们有希望增强牙科基质和改善材料的附着力。然而，在支持它们在临床应用之前，还需要更多的研究。目的：研究槲皮素和白藜芦醇对牙本质胶原原纤维结合强度和抗酶降解的影响。材料和方法：脱矿后的人牙本质标本分别在槲皮素或白藜芦醇（100、250和500µg/mL）、2%氯己定（CHX）或0.9%生理盐水（对照）溶液中浸泡1 min。37℃下用胶原酶孵育24 h，测定干质量变化。将槲皮素、白藜芦醇、CHX或蒸馏水（DW）涂于磷酸条件下的牙本质上，然后涂上Single bond 2 （3 M/ESPE）粘合剂和Z350XT树脂（3 M/ESPE）进行微拉伸粘结强度测试。分别在蒸馏水中浸泡24 h和1年后测定粘接强度。数据分析采用Kruskal-Wallis和Dunn检验或方差分析，然后采用Tukey检验(p)结果：槲皮素处理的标本与CHX相比，体重显著增加（p 0.05）。1年后，只有DW组的粘结强度有明显下降（p = 0.001）。结论：槲皮素或白藜芦醇处理牙本质能有效地保持牙本质的黏附强度，保护胶原蛋白免受酶降解，提示其具有提高牙本质黏附修复体寿命的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Clinical and Experimental Dental Research DENTISTRY, ORAL SURGERY & MEDICINE-

CiteScore

3.30

自引率

5.60%

发文量

165

审稿时长

26 weeks

期刊介绍： Clinical and Experimental Dental Research aims to provide open access peer-reviewed publications of high scientific quality representing original clinical, diagnostic or experimental work within all disciplines and fields of oral medicine and dentistry. The scope of Clinical and Experimental Dental Research comprises original research material on the anatomy, physiology and pathology of oro-facial, oro-pharyngeal and maxillofacial tissues, and functions and dysfunctions within the stomatognathic system, and the epidemiology, aetiology, prevention, diagnosis, prognosis and therapy of diseases and conditions that have an effect on the homeostasis of the mouth, jaws, and closely associated structures, as well as the healing and regeneration and the clinical aspects of replacement of hard and soft tissues with biomaterials, and the rehabilitation of stomatognathic functions. Studies that bring new knowledge on how to advance health on the individual or public health levels, including interactions between oral and general health and ill-health are welcome.