Ex vivo Evaluation of Live-MB2 and CX-2 Biotic Strains on Inflammatory and Barrier-Related Biomarkers in a Human Skin Atopic Dermatitis-Like Model.

IF 2.5 4区医学 Q3 DERMATOLOGY

Clinical, Cosmetic and Investigational Dermatology Pub Date : 2026-09-08 eCollection Date: 2026-01-01 DOI:10.2147/CCID.S630833

Sejin Cheon, Haeun Lee, Da Yeong Nam, Seokjun Kim, Kyeongeun Kim, Jaeyu Park, Dong-Geol Lee, Chun Ho Park, So Min Kang, Young Mok Heo, Hyun Ho Han, Dong Keon Yon

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引用次数: 0

Abstract

Objective: Atopic dermatitis (AD) is a chronic inflammatory skin condition characterized by epidermal barrier disruption and immune dysregulation. Live-MB2 and CX-2 were used in an ex vivo human skin AD-like model, which provides a biologically relevant platform for assessing cosmetic ingredients targeting AD-like features.

Methods: An AD-like condition was induced in ex vivo human skin explants by repeated tape stripping followed by 2,4-dinitrochlorobenzene stimulation. Skin tissues were treated with Live-MB2 or CX-2 via topical or dermal exposure. Epidermal thickness, IgE, and filaggrin levels were evaluated using hematoxylin and eosin, immunofluorescence, and Enzyme-Linked Immunosorbent Assay analyses.

Results: Topical application of Live-MB2 and CX-2 significantly reduced epidermal thickness compared with AD-induced controls. IgE levels were significantly reduced, while filaggrin expression indicated a marked increase, indicating barrier recovery. No treatment-related tissue damage was observed.

Conclusion: Live-MB2 and CX-2 showed favorable effects on inflammatory and barrier-related parameters in an ex vivo human skin AD-like model, supporting their potential application as cosmetic ingredients for sensitive and atopic-prone skin.

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活体mb2和CX-2生物菌株对人类皮肤特应性皮炎样模型炎症和屏障相关生物标志物的体外评估

目的：特应性皮炎（AD）是一种以表皮屏障破坏和免疫失调为特征的慢性炎症性皮肤病。Live-MB2和CX-2用于离体人皮肤ad样模型，为评估针对ad样特征的化妆品成分提供了生物学相关的平台。方法：采用重复撕带法和2,4-二硝基氯苯刺激法，在离体人体皮肤外植体中诱导ad样病变。皮肤组织用Live-MB2或CX-2局部或真皮暴露处理。使用苏木精和伊红、免疫荧光和酶联免疫吸附分析评估表皮厚度、IgE和聚丝蛋白水平。结果：与ad诱导的对照组相比，局部应用Live-MB2和CX-2可显著降低表皮厚度。IgE水平明显降低，而聚丝蛋白表达明显增加，表明屏障恢复。未观察到与治疗相关的组织损伤。结论：Live-MB2和CX-2在离体人皮肤ad样模型中对炎症和屏障相关参数有良好的影响，支持其作为敏感和易发特应性皮肤的化妆品成分的潜在应用。

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来源期刊

Clinical, Cosmetic and Investigational Dermatology Medicine-Dermatology

CiteScore

2.80

自引率

4.30%

发文量

353

审稿时长

16 weeks

期刊介绍： Clinical, Cosmetic and Investigational Dermatology is an international, peer-reviewed, open access journal that focuses on the latest clinical and experimental research in all aspects of skin disease and cosmetic interventions. Normal and pathological processes in skin development and aging, their modification and treatment, as well as basic research into histology of dermal and dermal structures that provide clinical insights and potential treatment options are key topics for the journal. Patient satisfaction, preference, quality of life, compliance, persistence and their role in developing new management options to optimize outcomes for target conditions constitute major areas of interest. The journal is characterized by the rapid reporting of clinical studies, reviews and original research in skin research and skin care. All areas of dermatology will be covered; contributions will be welcomed from all clinicians and basic science researchers globally.