Sex Differences in Angiogenic Ligand and Receptor Protein Expression During Adipose Tissue Expansion and Regression.

IF 3.5 4区医学 Q3 BIOPHYSICS

Cellular and molecular bioengineering Pub Date : 2026-07-01 eCollection Date: 2026-08-01 DOI:10.1007/s12195-026-00925-x

Yingye Fang, Silvia Gonzalez-Nieves, Vincenza Cifarelli, Princess I Imoukhuede

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引用次数: 0

Abstract

Purpose: Inadequate angiogenesis in obesogenic adipose tissue (AT) has been implicated in disrupted adipogenesis and metabolic disorders. While several regulators of AT angiogenesis have been identified, our understanding of the quantitative changes in angiogenic signaling proteins during obesity progression remains incomplete, particularly regarding sex-specifi c responses. This study sought to identify the dysregulated elements within the Vascular Endothelial Growth Factor (VEGF) and Platelet-Derived Growth Factor (PDGF) systems during obesity progression.

Methods: We employ a mouse model, comprising both male and female mice, to investigate the changes in the VEGF/PDGF concentration and their receptor distribution in gonadal AT during short- and long-term weight gain and weight loss.

Results: Female mice preserve adipose tissue angiogenic signatures during obesity progression, including sustained upregulation of endothelial VEGFR1 protein, maintenance of VEGF-A levels and endothelial cell populations, and differential regulation of PDGF levels, compared to males. These sex-specifi c patterns correlate with improved adipose expandability and may contribute to the more metabolically healthy obesity phenotype commonly observedin females.

Conclusion: These sex-specifi c patterns correlate with improved adipose expandability and may contribute to the more metabolically healthy obesity phenotype commonly observed in females. Our quantitative profi ling also lays the groundwork for developing computational models of VEGF/PDGF signaling networks in AT, allowing for the simulation of complex biological interactions and the prediction of therapeutic outcomes.

Supplementary information: The online version contains supplementary material available at https://doi.org/10.1007/s12195-026-00925-x.

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脂肪组织扩张和消退过程中血管生成配体和受体蛋白表达的性别差异。

目的：致肥性脂肪组织（AT）血管生成不足与脂肪生成中断和代谢紊乱有关。虽然已经确定了几个AT血管生成的调节因子，但我们对肥胖进展过程中血管生成信号蛋白的定量变化的理解仍然不完整，特别是关于性别特异性反应。本研究旨在确定肥胖进展过程中血管内皮生长因子（VEGF）和血小板衍生生长因子（PDGF）系统中的失调因子。方法：采用雄性和雌性小鼠模型，研究短期和长期增重和减重过程中生殖腺AT中VEGF/PDGF浓度及其受体分布的变化。结果：与雄性小鼠相比，雌性小鼠在肥胖进展过程中保留脂肪组织血管生成特征，包括内皮VEGFR1蛋白的持续上调，VEGF-A水平和内皮细胞群的维持，以及PDGF水平的差异调节。这些性别特异性模式与脂肪可扩展性的改善相关，并可能导致在女性中普遍观察到的代谢更健康的肥胖表型。结论：这些性别特异性模式与脂肪可扩展性的改善相关，可能有助于在女性中观察到的代谢更健康的肥胖表型。我们的定量分析也为开发AT中VEGF/PDGF信号网络的计算模型奠定了基础，允许模拟复杂的生物相互作用和预测治疗结果。补充信息：在线版本包含补充资料，可在https://doi.org/10.1007/s12195-026-00925-x上获得。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cellular and molecular bioengineering 生物-生物物理

CiteScore

5.60

自引率

3.60%

发文量

审稿时长

>12 weeks

期刊介绍： The field of cellular and molecular bioengineering seeks to understand, so that we may ultimately control, the mechanical, chemical, and electrical processes of the cell. A key challenge in improving human health is to understand how cellular behavior arises from molecular-level interactions. CMBE, an official journal of the Biomedical Engineering Society, publishes original research and review papers in the following seven general areas: Molecular: DNA-protein/RNA-protein interactions, protein folding and function, protein-protein and receptor-ligand interactions, lipids, polysaccharides, molecular motors, and the biophysics of macromolecules that function as therapeutics or engineered matrices, for example. Cellular: Studies of how cells sense physicochemical events surrounding and within cells, and how cells transduce these events into biological responses. Specific cell processes of interest include cell growth, differentiation, migration, signal transduction, protein secretion and transport, gene expression and regulation, and cell-matrix interactions. Mechanobiology: The mechanical properties of cells and biomolecules, cellular/molecular force generation and adhesion, the response of cells to their mechanical microenvironment, and mechanotransduction in response to various physical forces such as fluid shear stress. Nanomedicine: The engineering of nanoparticles for advanced drug delivery and molecular imaging applications, with particular focus on the interaction of such particles with living cells. Also, the application of nanostructured materials to control the behavior of cells and biomolecules.