Qufeng Zhijing Capsule attenuates airway inflammation in experimental asthma and is associated with c-Myc/YTHDF1/TRPV1-related signaling

IF 3.5 4区综合性期刊 Q2 MULTIDISCIPLINARY SCIENCES

Journal of Radiation Research and Applied Sciences Pub Date : 2026-09-01 Epub Date: 2026-08-08 DOI:10.1016/j.jrras.2026.102607

Yugang Wu, Jie Wen, Juanli Liu, Jianjiang Luo

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引用次数: 0

Abstract

Background

Qufeng Zhijing Capsule (QFZJ) is a traditional Chinese medicine formulation used for asthma-related respiratory symptoms, but its effects on airway epithelial inflammation and the underlying molecular mechanisms remain unclear.

Methods

OVA-induced asthmatic rats received QFZJ at 0.5, 1.0, or 2.0 g/kg/day. BEAS-2B cells were pretreated with QFZJ-containing serum before LPS stimulation. Airway inflammation, cell viability, apoptosis, intracellular Ca²⁺ responses, molecular expression, c-Myc enrichment at the YTHDF1 promoter, and TRPV1 mRNA m6A enrichment were evaluated.

Results

Medium-dose QFZJ produced the most consistent improvement in lung pathology and reduced inflammatory cytokines and epithelial alarmins in OVA-challenged rats. QFZJ also decreased the expression of c-Myc, YTHDF1, TRPV1, c-Fos, and c-Jun. In LPS-stimulated BEAS-2B cells, QFZJ-containing serum improved cell viability, reduced apoptosis and inflammatory mediator release, and attenuated intracellular Ca²⁺ responses. Overexpression of c-Myc or TRPV1 partially diminished these effects. ChIP-qPCR showed enrichment of c-Myc at a predicted region of the YTHDF1 promoter, whereas m6A-RIP-qPCR showed that QFZJ reduced LPS-induced m6A enrichment of TRPV1 mRNA.

Conclusion

These findings support the involvement of c-Myc/YTHDF1/TRPV1-related signaling in the protective effects of QFZJ against airway epithelial inflammation. However, further loss-of-function and direct molecular interaction studies are required to establish the causal and hierarchical relationships within this pathway.

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祛风止净胶囊可减轻实验性哮喘气道炎症，并与c-Myc/YTHDF1/ trpv1相关信号通路相关

祛风止净胶囊是一种用于治疗哮喘相关呼吸道症状的中药制剂，但其对气道上皮炎症的影响及其分子机制尚不清楚。方法sova诱导的哮喘大鼠按0.5、1.0、2.0 g/kg/d给药。在LPS刺激前，用含qfzj的血清预处理BEAS-2B细胞。评估气道炎症、细胞活力、细胞凋亡、细胞内Ca2+反应、分子表达、YTHDF1启动子c-Myc富集和TRPV1 mRNA m6A富集。结果中剂量清风津对ova大鼠肺病理的改善效果最一致，炎症因子和上皮因子的减少最为明显。QFZJ还能降低c-Myc、YTHDF1、TRPV1、c-Fos和c-Jun的表达。在lps刺激的BEAS-2B细胞中，含qfzj的血清可提高细胞活力，减少细胞凋亡和炎症介质释放，并减弱细胞内Ca2+反应。c-Myc或TRPV1的过表达部分减弱了这些作用。ChIP-qPCR显示c-Myc在YTHDF1启动子的预测区域富集，而m6A- rip - qpcr显示QFZJ降低了lps诱导的m6A对TRPV1 mRNA的富集。结论本研究结果支持c-Myc/YTHDF1/ trpv1相关信号通路参与芪芪清注射液对气道上皮炎症的保护作用。然而，需要进一步的功能丧失和直接的分子相互作用研究来建立这一途径中的因果关系和层次关系。

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来源期刊

Journal of Radiation Research and Applied Sciences MULTIDISCIPLINARY SCIENCES-

自引率

5.90%

发文量

130

审稿时长

16 weeks

期刊介绍： Journal of Radiation Research and Applied Sciences provides a high quality medium for the publication of substantial, original and scientific and technological papers on the development and applications of nuclear, radiation and isotopes in biology, medicine, drugs, biochemistry, microbiology, agriculture, entomology, food technology, chemistry, physics, solid states, engineering, environmental and applied sciences.