FLInt in C. briggsae and C. tropicalis : Strains and resources for fast and locus-targeted integration of multi-copy transgenes.

Abstract

C. briggsae and C. tropicalis are satellite model systems for molecular mechanistic investigations and for comparative studies across Caenorhabditis species. FLInt ( F luorescent L andmark Int erference) was developed in C. elegans for rapid generation of transgenic strains through integration of multi-copy DNA arrays into predetermined genomic loci, without requiring a random mutagenesis step. Here, we present resources and newly generated strains that enable FLInt in C. briggsae and C. tropicalis , yielding integrated transgenic lines within ~10 days of microinjection.