Zar Chi Soe, Daneeya Na Nan, Rahman Wahyudi, Kanokwan Sriwattanapong, Thantrira Porntaveetus, Soranun Chantarangsu, Paksinee Kamolratanakul, Vincent Everts, Chatvadee Kornsuthisopon, Thanaphum Osathanon, Sornkanok Vimolmangkang, Nuttapol Limjeerajarus, Chalida Nakalekha Limjeerajarus
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引用次数: 0
Abstract
Introduction: Dental pulp inflammation, caused by caries, trauma, or restorative operations, produces pain and slows regeneration. Cannabidiol (CBD) and Δ9-tetrahydrocannabinol (THC), cannabinoids of Cannabis sativa, have effects on inflammation and pain relief. However, the impact of these factors on dental pulp healing is not yet understood. This study investigated the combined effects of CBD and THC in controlling inflammation and nociception during dental pulp repair.
Methods: Human dental pulp cells were stimulated with lipopolysaccharide and treated with CBD (2.5 μg/mL), THC (0.6 μg/mL), and their combination. Pro-inflammatory cytokines (interleukin [IL]-6, IL-12, and IL-1β) and anti-inflammatory cytokine IL-10 were analyzed using qPCR and enzyme-linked immunosorbent assay, while anti-nociceptive markers (cannabinoid receptor type 1, cannabinoid receptor type 2, and transient receptor potential vanilloid 1) were evaluated by qPCR and Western blot. Cell viability, proliferation, and migration assays were performed to evaluate treatment effects on cellular behaviors associated with pulpal repair and regenerative potential.
Results: Compared with either treatment alone, the CBD/THC combination more effectively attenuated pro-inflammatory cytokines IL-6, IL-12, and IL-1β, enhanced cannabinoid receptor type 1 and cannabinoid receptor type 2 expression, modulated transient receptor potential vanilloid 1, and increased IL-10 expression. Viability assays determined optimal non-cytotoxic concentrations for treatment. The combination enhanced cell proliferation and migration, promoted mineralized nodule formation, and significantly upregulated markers associated with tissue repair, odontogenic differentiation (dentin matrix protein 1), and angiogenesis (vascular endothelial growth factor and basic fibroblast growth factor).
Conclusion: Combined CBD and THC show promise for promoting dental pulp healing by modulating inflammation and nociceptive signaling, while supporting odontogenic differentiation, angiogenesis, and cell migration in human dental pulp cells. These findings provide in vitro evidence supporting combined cannabinoid treatment as a therapeutic strategy for vital pulp therapies.
期刊介绍：
The Journal of Endodontics, the official journal of the American Association of Endodontists, publishes scientific articles, case reports and comparison studies evaluating materials and methods of pulp conservation and endodontic treatment. Endodontists and general dentists can learn about new concepts in root canal treatment and the latest advances in techniques and instrumentation in the one journal that helps them keep pace with rapid changes in this field.
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