{"title":"SCULPT-MCP: Systematic Chromosomal Upgrades for Locus-Driven Protein Topologies in Microcompartments","authors":"Dimple Goel, Preeti Negi, Aarcha Radhakrishnan, Sharmistha Sinha","doi":"10.1021/acschembio.6c00504","DOIUrl":null,"url":null,"abstract":"<p>Gene order is a powerful design principle for protein nanomachines. In nature, gene organization ensures the precise assembly of functional protein nanostructures. We demonstrate how the genetic repositioning of the key structural gene <em>pduN</em> within the operon encoding a self-assembling protein nanocompartment sculpts the morphology and function of bacterial microcompartments (BMCs). Relocating <em>pduN</em> to new operonic positions dramatically altered the size, shape, and catalytic output of BMCs, despite identical protein sequences. These shifts reveal how gene order may control nanoscale assembly and compartmentalized function. Our findings establish operon architecture as a programmable genetic framework for nanostructure morphogenesis and provide a synthetic biology strategy to engineer self-assembling nanodevices with customized geometries and activities.</p>","PeriodicalId":11,"journal":{"name":"ACS Chemical Biology","volume":"21 10","pages":"2662–2674"},"PeriodicalIF":3.6000,"publicationDate":"2026-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS Chemical Biology","FirstCategoryId":"99","ListUrlMain":"https://pubs.acs.org/doi/10.1021/acschembio.6c00504","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2026/9/10 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Gene order is a powerful design principle for protein nanomachines. In nature, gene organization ensures the precise assembly of functional protein nanostructures. We demonstrate how the genetic repositioning of the key structural gene pduN within the operon encoding a self-assembling protein nanocompartment sculpts the morphology and function of bacterial microcompartments (BMCs). Relocating pduN to new operonic positions dramatically altered the size, shape, and catalytic output of BMCs, despite identical protein sequences. These shifts reveal how gene order may control nanoscale assembly and compartmentalized function. Our findings establish operon architecture as a programmable genetic framework for nanostructure morphogenesis and provide a synthetic biology strategy to engineer self-assembling nanodevices with customized geometries and activities.
期刊介绍：
ACS Chemical Biology provides an international forum for the rapid communication of research that broadly embraces the interface between chemistry and biology.
The journal also serves as a forum to facilitate the communication between biologists and chemists that will translate into new research opportunities and discoveries. Results will be published in which molecular reasoning has been used to probe questions through in vitro investigations, cell biological methods, or organismic studies.
We welcome mechanistic studies on proteins, nucleic acids, sugars, lipids, and nonbiological polymers. The journal serves a large scientific community, exploring cellular function from both chemical and biological perspectives. It is understood that submitted work is based upon original results and has not been published previously.
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