Combined Expression of Cyclin B1 and Cytoplasmic HMGB1 Is Associated With Delayed Cervical Lymph Node Metastasis in Early-Stage Tongue Squamous Cell Carcinoma.

IF 3.1 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Oral diseases Pub Date : 2026-09-08 DOI:10.1111/odi.70476

Wataru Kakuguchi, Aya Yanagawa-Matsuda, Nako Maishi, Takehiro Chida, Kenta Takahashi, Shoko Kirikoshi, Kyoko Hida, Yuichi Ashikaga, Yoichi Ohiro

{"title":"Combined Expression of Cyclin B1 and Cytoplasmic HMGB1 Is Associated With Delayed Cervical Lymph Node Metastasis in Early-Stage Tongue Squamous Cell Carcinoma.","authors":"Wataru Kakuguchi, Aya Yanagawa-Matsuda, Nako Maishi, Takehiro Chida, Kenta Takahashi, Shoko Kirikoshi, Kyoko Hida, Yuichi Ashikaga, Yoichi Ohiro","doi":"10.1111/odi.70476","DOIUrl":null,"url":null,"abstract":"Objective: Delayed cervical lymph node metastasis is strongly associated with poor prognosis in early-stage tongue squamous cell carcinoma, but useful biomarkers remain limited. This study evaluated cyclin B1, high-mobility group box protein 1 and 2, and proliferating cell nuclear antigen for stratifying the risk of delayed cervical lymph node metastasis.Methods: Eighty-two patients with Stage I/II tongue squamous cell carcinoma who underwent partial glossectomy without elective neck dissection were included. Cyclin B1 and the cytoplasmic expression of the other three markers were assessed immunohistochemically. Cutoff values were determined using receiver operating characteristic analysis. Cervical lymph node metastasis-free survival was estimated using Kaplan-Meier and multivariable Cox analyses.Results: Cyclin B1 showed the strongest association with delayed cervical lymph node metastasis (χ2 = 29.0, p < 0.0001; area under the curve, 0.80; sensitivity, 84.0%; specificity, 77.2%). Dual positivity for cyclin B1 and cytoplasmic high-mobility group box protein 1 showed 76.0% sensitivity and 87.7% specificity and remained significantly associated with metastasis-free survival after adjustment for pathological depth of invasion and Yamamoto-Kohama classification (adjusted hazard ratio, 8.57; 95% confidence interval, 3.44-24.36; p < 0.0001).Conclusions: Combined cyclin B1 and cytoplasmic high-mobility group box protein 1 assessment may aid postoperative risk stratification.","PeriodicalId":19615,"journal":{"name":"Oral diseases","volume":" ","pages":""},"PeriodicalIF":3.1000,"publicationDate":"2026-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Oral diseases","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1111/odi.70476","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"DENTISTRY, ORAL SURGERY & MEDICINE","Score":null,"Total":0}

引用次数: 0

Abstract

Objective: Delayed cervical lymph node metastasis is strongly associated with poor prognosis in early-stage tongue squamous cell carcinoma, but useful biomarkers remain limited. This study evaluated cyclin B1, high-mobility group box protein 1 and 2, and proliferating cell nuclear antigen for stratifying the risk of delayed cervical lymph node metastasis.

Methods: Eighty-two patients with Stage I/II tongue squamous cell carcinoma who underwent partial glossectomy without elective neck dissection were included. Cyclin B1 and the cytoplasmic expression of the other three markers were assessed immunohistochemically. Cutoff values were determined using receiver operating characteristic analysis. Cervical lymph node metastasis-free survival was estimated using Kaplan-Meier and multivariable Cox analyses.

Results: Cyclin B1 showed the strongest association with delayed cervical lymph node metastasis (χ² = 29.0, p < 0.0001; area under the curve, 0.80; sensitivity, 84.0%; specificity, 77.2%). Dual positivity for cyclin B1 and cytoplasmic high-mobility group box protein 1 showed 76.0% sensitivity and 87.7% specificity and remained significantly associated with metastasis-free survival after adjustment for pathological depth of invasion and Yamamoto-Kohama classification (adjusted hazard ratio, 8.57; 95% confidence interval, 3.44-24.36; p < 0.0001).

Conclusions: Combined cyclin B1 and cytoplasmic high-mobility group box protein 1 assessment may aid postoperative risk stratification.

查看原文本刊更多论文

细胞周期蛋白B1和细胞质HMGB1的联合表达与早期舌鳞癌迟发性颈淋巴结转移相关

目的：延迟颈部淋巴结转移与早期舌鳞癌预后不良密切相关，但有用的生物标志物仍然有限。本研究评估了细胞周期蛋白B1、高迁移率组盒蛋白1和2以及增殖细胞核抗原对迟发性颈淋巴结转移风险的分层作用。方法：82例ⅰ/ⅱ期舌鳞癌患者行部分舌骨切除术，未择期颈部清扫。免疫组织化学检测细胞周期蛋白B1及其他3种标志物的细胞质表达。利用接收机工作特性分析确定截止值。使用Kaplan-Meier和多变量Cox分析估计颈部淋巴结无转移生存率。结果：细胞周期蛋白B1与迟发性颈淋巴转移相关性最强（χ2 = 29.0, p）。结论：细胞周期蛋白B1与细胞质高迁移率组盒蛋白1联合评估有助于术后风险分层。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Oral diseases 医学-牙科与口腔外科

CiteScore

7.60

自引率

5.30%

发文量

325

审稿时长

4-8 weeks

期刊介绍： Oral Diseases is a multidisciplinary and international journal with a focus on head and neck disorders, edited by leaders in the field, Professor Giovanni Lodi (Editor-in-Chief, Milan, Italy), Professor Stefano Petti (Deputy Editor, Rome, Italy) and Associate Professor Gulshan Sunavala-Dossabhoy (Deputy Editor, Shreveport, LA, USA). The journal is pre-eminent in oral medicine. Oral Diseases specifically strives to link often-isolated areas of dentistry and medicine through broad-based scholarship that includes well-designed and controlled clinical research, analytical epidemiology, and the translation of basic science in pre-clinical studies. The journal typically publishes articles relevant to many related medical specialties including especially dermatology, gastroenterology, hematology, immunology, infectious diseases, neuropsychiatry, oncology and otolaryngology. The essential requirement is that all submitted research is hypothesis-driven, with significant positive and negative results both welcomed. Equal publication emphasis is placed on etiology, pathogenesis, diagnosis, prevention and treatment.