Collagen Substitutes in Oral Soft Tissue Augmentation: An In Vitro–Ex Vivo Study on Expansion and Elasticity

IF 2.4 Q3 DENTISTRY, ORAL SURGERY & MEDICINE

Clinical and Experimental Dental Research Pub Date : 2026-09-08 DOI:10.1002/cre2.70448

Leonardo Mancini, Stephanie Wenk, Lorenzo Tavelli, Ronald E. Jung, Nadja Naenni, Daniel S. Thoma

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引用次数: 0

Abstract

Objective

This study aimed to evaluate the hydration-induced volumetric expansion and elasticity of collagen-based soft tissue substitutes following exposure to blood using three-dimensional (3D) analysis and high-frequency ultrasonography (HFUS).

Materials and Methods

Seven commercially available collagen matrices were analyzed: Fibro-Gide (6 mm and 3 mm), Mucogain (5 mm and 3 mm), Volumax, Collagen Graft II, and Mucograft. The investigation consisted of two experimental phases. In the in vitro phase, samples were immersed in stabilized porcine blood to assess absorption index (AI), 3D volumetric changes, and ultrasonographic (HFUS) parameters, including density and elasticity. In the ex vivo phase, matrices were placed beneath envelope flaps in porcine maxillae, with optical 3D scanning performed before and after blood saturation. Statistical analysis included generalized estimating equations for repeated measures and nonparametric testing, with significance set at α = 0.05.

Results

Volume stable matrices exhibited significantly greater absorption and volumetric expansion compared to non-stable matrices. Fibro-Gide 6 mm demonstrated the highest absorption index (17.0 ± 0.6 mg) and volumetric increase (268.4 ± 54.8 mm³), while Mucogain 5 mm showed the greatest vertical expansion (0.83 ± 0.08 mm). Non-stable matrices displayed reduced expansion or partial collapse. A positive, though non-significant, correlation was observed between density (HFUS) and volumetric expansion (r = 0.29, p = 0.535).

Conclusions

Volume-stable collagen matrices show pronounced fluid uptake and expansion. Clinically, this effect should be considered, as excessive swelling may increase flap tension and compromise wound stability, necessitating careful trimming and adequate flap release.

查看原文本刊更多论文

胶原替代物在口腔软组织增强中的应用：扩张和弹性的体内外研究。

目的：本研究旨在利用三维（3D）分析和高频超声（HFUS）技术评估胶原基软组织替代物暴露于血液后水合诱导的体积膨胀和弹性。材料和方法：分析了七种市售胶原基质：fiber - gide (6mm和3mm), Mucogain (5mm和3mm), Volumax， collagen Graft II和Mucograft。调查包括两个实验阶段。在体外阶段，将样品浸入稳定的猪血中，评估吸收指数（AI）、三维体积变化和超声（HFUS）参数，包括密度和弹性。在离体阶段，将基质放置在猪上颌骨的包膜瓣下，在血液饱和前后进行光学3D扫描。统计分析包括重复测量的广义估计方程和非参数检验，显著性设置为α = 0.05。结果：相对于非稳定基质，体积稳定基质表现出更大的吸收和体积膨胀。纤维- gide 6 mm吸收指数最高（17.0±0.6 mg），体积增大（268.4±54.8 mm3）， Mucogain 5 mm垂直扩张最大（0.83±0.08 mm）。非稳定矩阵表现为缩胀或部分塌缩。密度（HFUS）与体积膨胀呈正相关，但不显著（r = 0.29, p = 0.535）。结论：体积稳定的胶原基质表现出明显的液体摄取和扩张。在临床上，应考虑到这种影响，因为过度肿胀可能会增加皮瓣张力，损害伤口稳定性，需要仔细修剪和适当的皮瓣释放。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Clinical and Experimental Dental Research DENTISTRY, ORAL SURGERY & MEDICINE-

CiteScore

3.30

自引率

5.60%

发文量

165

审稿时长

26 weeks

期刊介绍： Clinical and Experimental Dental Research aims to provide open access peer-reviewed publications of high scientific quality representing original clinical, diagnostic or experimental work within all disciplines and fields of oral medicine and dentistry. The scope of Clinical and Experimental Dental Research comprises original research material on the anatomy, physiology and pathology of oro-facial, oro-pharyngeal and maxillofacial tissues, and functions and dysfunctions within the stomatognathic system, and the epidemiology, aetiology, prevention, diagnosis, prognosis and therapy of diseases and conditions that have an effect on the homeostasis of the mouth, jaws, and closely associated structures, as well as the healing and regeneration and the clinical aspects of replacement of hard and soft tissues with biomaterials, and the rehabilitation of stomatognathic functions. Studies that bring new knowledge on how to advance health on the individual or public health levels, including interactions between oral and general health and ill-health are welcome.