Urine and Serum Proteome and Lipidome Analysis of Naturally Aging Feline Species

IF 3.8 2区生物学 Q1 BIOCHEMICAL RESEARCH METHODS

Journal of Proteome Research Pub Date : 2026-09-04 Epub Date: 2026-08-11 DOI:10.1021/acs.jproteome.6c00247

Punyatoya Panda, Rodrigo Mohallem, Rita de Kassia Rodrigues Bezerra Filgueira, Niwako Ogata, Uma K. Aryal

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Abstract

Aging in companion animals such as cats closely relates to human aging in environmental exposures and disease manifestation, providing a valuable model for identifying biomarkers of age-associated decline. This study provides a combined proteomic and lipidomic analysis of serum and urine from naturally aging domestic cats aged 3.8–16 years, grouped as adult, old, and senior, to identify age-related molecular changes across biofluids. Label-free quantitative proteomics identified 901 urinary and 238 serum proteins, with 75 urinary proteins significantly altered with age that are linked to kidney disease, hypertension, neurodegeneration, and metabolic disorders. In contrast, only six serum proteins differed significantly between adult and old/senior cats, including decreased Apolipoprotein A-I (APOA1) in seniors, a protein linked with cognitive function in aging. Untargeted lipidomics revealed increases in specific serum triacylglycerols, phosphatidylcholines (PCs), and sphingomyelins, while urinary lipid profiles showed limited age-related changes, with some PCs decreasing, and diacylglycerols increasing with age. These results demonstrate distinct systemic and renal molecular remodeling during feline aging and highlight the utility of integrated omics analyses of biological fluids for identifying molecular alterations relevant to both feline and human aging.

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自然衰老猫科动物尿液和血清蛋白质组和脂质组分析。

伴侣动物（如猫）的衰老与人类在环境暴露和疾病表现方面的衰老密切相关，为识别年龄相关衰退的生物标志物提供了有价值的模型。本研究对3.8-16岁自然衰老的家猫（分为成年、老年和老年）的血清和尿液进行了蛋白质组学和脂质组学分析，以确定生物体液中与年龄相关的分子变化。无标记定量蛋白质组学鉴定了901种尿蛋白和238种血清蛋白，其中75种尿蛋白随年龄显著改变，与肾脏疾病、高血压、神经变性和代谢紊乱有关。相比之下，只有6种血清蛋白在成年猫和老年猫之间存在显著差异，包括老年猫的载脂蛋白a - i （APOA1）减少，这是一种与衰老过程中认知功能相关的蛋白质。非靶向脂质组学显示特异性血清甘油三酯、磷脂酰胆碱（PCs）和鞘磷脂增加，而尿脂谱显示有限的年龄相关变化，一些PCs减少，二酰基甘油随年龄增加。这些结果证明了猫衰老过程中不同的系统和肾脏分子重塑，并强调了生物流体集成组学分析在识别与猫和人类衰老相关的分子改变方面的实用性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Proteome Research 生物-生化研究方法

CiteScore

9.00

自引率

4.50%

发文量

251

审稿时长

3 months

期刊介绍： Journal of Proteome Research publishes content encompassing all aspects of global protein analysis and function, including the dynamic aspects of genomics, spatio-temporal proteomics, metabonomics and metabolomics, clinical and agricultural proteomics, as well as advances in methodology including bioinformatics. The theme and emphasis is on a multidisciplinary approach to the life sciences through the synergy between the different types of "omics".