Sulfonation modification of REDV peptides for directing cardiovascular cell fates.

IF 6 2区医学 Q2 MATERIALS SCIENCE, BIOMATERIALS

Materials Science & Engineering C-Materials for Biological Applications Pub Date : 2026-09-01 DOI:10.1016/j.bioadv.2026.215157

Zhe Fang, Shuaiwei Xu, Fan Li, Siyu Liu, Huimin Duan, Yanchao Wang, Hao Sun, Jingan Li, Shaokang Guan

{"title":"Sulfonation modification of REDV peptides for directing cardiovascular cell fates.","authors":"Zhe Fang, Shuaiwei Xu, Fan Li, Siyu Liu, Huimin Duan, Yanchao Wang, Hao Sun, Jingan Li, Shaokang Guan","doi":"10.1016/j.bioadv.2026.215157","DOIUrl":null,"url":null,"abstract":"<p><p>The Arginine-Glutamic acid-Aspartic acid-Valine (Arg-Glu-Asp-Val, REDV) peptide selectively binds endothelial cells but exhibits limited multifunctional bioactivity for cardiovascular applications. Sulfonation has been reported to confer multi-cellular regulatory functions to various biomolecules. In this study, sulfonated REDV peptides (S-REDV) with sulfur contents of 3.56 ± 0.10, 4.20 ± 0.23, 5.39 ± 0.13, and 5.98 ± 0.08 at.% were prepared by controlling the reaction time. Comprehensive cytocompatibility evaluations revealed that a moderate sulfonation degree (S-REDV-3) significantly enhanced human umbilical vein endothelial cell (HUVEC) proliferation, nitric oxide (NO) release and migration. Moreover, it restrained excessive smooth muscle cell (SMC) proliferation, preserved the contractile phenotype of SMCs, and drove macrophage polarization toward an anti-inflammatory phenotype. In contrast, excessive sulfonation resulted in structural degradation and compromised bioactivity. These results indicate that appropriately sulfonated REDV, particularly S-REDV-3, may serve as a promising bioactive peptide for surface functionalization of cardiovascular biomaterials.</p>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"190 ","pages":"215157"},"PeriodicalIF":6.0000,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Materials Science & Engineering C-Materials for Biological Applications","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1016/j.bioadv.2026.215157","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MATERIALS SCIENCE, BIOMATERIALS","Score":null,"Total":0}

引用次数: 0

Abstract

The Arginine-Glutamic acid-Aspartic acid-Valine (Arg-Glu-Asp-Val, REDV) peptide selectively binds endothelial cells but exhibits limited multifunctional bioactivity for cardiovascular applications. Sulfonation has been reported to confer multi-cellular regulatory functions to various biomolecules. In this study, sulfonated REDV peptides (S-REDV) with sulfur contents of 3.56 ± 0.10, 4.20 ± 0.23, 5.39 ± 0.13, and 5.98 ± 0.08 at.% were prepared by controlling the reaction time. Comprehensive cytocompatibility evaluations revealed that a moderate sulfonation degree (S-REDV-3) significantly enhanced human umbilical vein endothelial cell (HUVEC) proliferation, nitric oxide (NO) release and migration. Moreover, it restrained excessive smooth muscle cell (SMC) proliferation, preserved the contractile phenotype of SMCs, and drove macrophage polarization toward an anti-inflammatory phenotype. In contrast, excessive sulfonation resulted in structural degradation and compromised bioactivity. These results indicate that appropriately sulfonated REDV, particularly S-REDV-3, may serve as a promising bioactive peptide for surface functionalization of cardiovascular biomaterials.

查看原文本刊更多论文

用于指导心血管细胞命运的REDV肽磺化修饰。

精氨酸-谷氨酸-天冬氨酸-缬氨酸（Arg-Glu-Asp-Val， REDV）肽选择性地结合内皮细胞，但在心血管应用中表现出有限的多功能生物活性。磺化已被报道赋予多种生物分子多细胞调节功能。在本研究中，磺化的REDV肽（S-REDV）的硫含量分别为3.56±0.10、4.20±0.23、5.39±0.13和5.98±0.08 at。通过控制反应时间制得。综合细胞相容性评价显示，适度磺化程度（S-REDV-3）可显著增强人脐静脉内皮细胞（HUVEC）的增殖、一氧化氮（NO）的释放和迁移。抑制平滑肌细胞过度增殖，保持平滑肌细胞的收缩表型，推动巨噬细胞向抗炎表型极化。相反，过度磺化会导致结构降解和生物活性受损。这些结果表明，适当磺化的REDV，特别是S-REDV-3，可能作为一种有前景的生物活性肽，用于心血管生物材料的表面功能化。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Materials Science & Engineering C-Materials for Biological Applications 工程技术-材料科学：生物材料

CiteScore

17.80

自引率

0.00%

发文量

501

审稿时长

27 days

期刊介绍： Biomaterials Advances, previously known as Materials Science and Engineering: C-Materials for Biological Applications (P-ISSN: 0928-4931, E-ISSN: 1873-0191). Includes topics at the interface of the biomedical sciences and materials engineering. These topics include: • Bioinspired and biomimetic materials for medical applications • Materials of biological origin for medical applications • Materials for "active" medical applications • Self-assembling and self-healing materials for medical applications • "Smart" (i.e., stimulus-response) materials for medical applications • Ceramic, metallic, polymeric, and composite materials for medical applications • Materials for in vivo sensing • Materials for in vivo imaging • Materials for delivery of pharmacologic agents and vaccines • Novel approaches for characterizing and modeling materials for medical applications Manuscripts on biological topics without a materials science component, or manuscripts on materials science without biological applications, will not be considered for publication in Materials Science and Engineering C. New submissions are first assessed for language, scope and originality (plagiarism check) and can be desk rejected before review if they need English language improvements, are out of scope or present excessive duplication with published sources. Biomaterials Advances sits within Elsevier''s biomaterials science portfolio alongside Biomaterials, Materials Today Bio and Biomaterials and Biosystems. As part of the broader Materials Today family, Biomaterials Advances offers authors rigorous peer review, rapid decisions, and high visibility. We look forward to receiving your submissions!