Synergistic Combination of Cryptolepis sanguinolenta (Lindl.) Schlechter (Apocynaceae) and Azadirachta Indica (A. Juss) (Meliaceae) Against 3D7 and DD2 Strains of Plasmodium Falciparum.

IF 2.9 Q3 PHARMACOLOGY & PHARMACY

Advances in Pharmacological and Pharmaceutical Sciences Pub Date : 2026-08-31 eCollection Date: 2026-01-01 DOI:10.1155/adpp/1273876

Samuel Korsah, Nathaniel Nene Djangmah Nortey, Christian Akakpo, Felix Kwame Zoiku, Jessica Korsah, Miriam Tagoe, Joseph Kwao Amanor, Babu Jacob Yamba, Josephine Akosua Ofori-Asamoah

{"title":"Synergistic Combination of Cryptolepis sanguinolenta (Lindl.) Schlechter (Apocynaceae) and Azadirachta Indica (A. Juss) (Meliaceae) Against 3D7 and DD2 Strains of Plasmodium Falciparum.","authors":"Samuel Korsah, Nathaniel Nene Djangmah Nortey, Christian Akakpo, Felix Kwame Zoiku, Jessica Korsah, Miriam Tagoe, Joseph Kwao Amanor, Babu Jacob Yamba, Josephine Akosua Ofori-Asamoah","doi":"10.1155/adpp/1273876","DOIUrl":null,"url":null,"abstract":"Introduction: Azadirachta indica and Cryptolepis sanguinolenta are herbs commonly used together in polyherbal products in Ghana for the treatment of malaria.Aim: This research investigated the combined effects of Azadirachta indica and Cryptolepis sanguinolenta extracts against chloroquine-sensitive (3D7) and resistant (DD2) strains of Plasmodium falciparum.Methods: In vitro antimalarial activity was assayed on the aqueous extracts of both plants using a fluorescence-based SYBR Green assay. The combined effects of the two extracts were determined using computer-based software, CompuSyn.Results: Aqueous extracts of Azadirachta indica and Cryptolepis sanguinolenta exerted high antimalarial activities (IC50 ≤ 10 μg/mL) against both 3D7 and DD2 strains of the Plasmodium falciparum. Azadirachta indica and Cryptolepis sanguinolenta displayed strong synergistic activity with combination index (CI) values ranging from 0.15 to 0.52 for 53%-99% inhibition of 3D7 Plasmodium falciparum. Also, CI values for the plants against DD2 were from 0.23 to 0.61 with corresponding 30%-97% parasitemia inhibitions. Azadirachta indica and Cryptolepis sanguinolenta exerted a dose reduction index (DRI) value greater than 1 (DRI > 1) in both strains.Conclusion: Cryptolepis sanguinolenta and Azadirachta indica exerted synergistic interactions against 3D7 and DD2 strains of Plasmodium falciparum. The extracts are desirable when used in combination rather than alone.","PeriodicalId":7369,"journal":{"name":"Advances in Pharmacological and Pharmaceutical Sciences","volume":"2026 ","pages":"1273876"},"PeriodicalIF":2.9000,"publicationDate":"2026-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13527865/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Advances in Pharmacological and Pharmaceutical Sciences","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1155/adpp/1273876","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2026/1/1 0:00:00","PubModel":"eCollection","JCR":"Q3","JCRName":"PHARMACOLOGY & PHARMACY","Score":null,"Total":0}

引用次数: 0

Abstract

Introduction: Azadirachta indica and Cryptolepis sanguinolenta are herbs commonly used together in polyherbal products in Ghana for the treatment of malaria.

Aim: This research investigated the combined effects of Azadirachta indica and Cryptolepis sanguinolenta extracts against chloroquine-sensitive (3D7) and resistant (DD2) strains of Plasmodium falciparum.

Methods: In vitro antimalarial activity was assayed on the aqueous extracts of both plants using a fluorescence-based SYBR Green assay. The combined effects of the two extracts were determined using computer-based software, CompuSyn.

Results: Aqueous extracts of Azadirachta indica and Cryptolepis sanguinolenta exerted high antimalarial activities (IC₅₀ ≤ 10 μg/mL) against both 3D7 and DD2 strains of the Plasmodium falciparum. Azadirachta indica and Cryptolepis sanguinolenta displayed strong synergistic activity with combination index (CI) values ranging from 0.15 to 0.52 for 53%-99% inhibition of 3D7 Plasmodium falciparum. Also, CI values for the plants against DD2 were from 0.23 to 0.61 with corresponding 30%-97% parasitemia inhibitions. Azadirachta indica and Cryptolepis sanguinolenta exerted a dose reduction index (DRI) value greater than 1 (DRI > 1) in both strains.

Conclusion: Cryptolepis sanguinolenta and Azadirachta indica exerted synergistic interactions against 3D7 and DD2 strains of Plasmodium falciparum. The extracts are desirable when used in combination rather than alone.

查看原文本刊更多论文

血隐霉的增效组合夹竹桃科和印楝科对恶性疟原虫3D7和DD2株的拮抗作用

导语：印楝和血樟是加纳用于治疗疟疾的复方草药产品中常用的草药。目的：研究印楝和血隐皮提取物对氯喹敏感（3D7）和耐药（DD2）恶性疟原虫的联合抑菌作用。方法：采用SYBR Green荧光法测定两种植物水提物的体外抗疟活性。两种提取物的综合效果是用计算机软件CompuSyn测定的。结果：印楝和血隐盖草水提物对恶性疟原虫3D7和DD2株均有较高的抗疟活性（IC50≤10 μg/mL）。印楝与血隐盖草对3D7型恶性疟原虫的抑制效果较好，联合指数（CI）在0.15 ~ 0.52之间，抑制效果为53% ~ 99%。对DD2的CI值为0.23 ~ 0.61，抑虫率为30% ~ 97%。印楝和血绿隐盖菌的剂量减少指数（DRI）均大于1 （DRI bbbb1）。结论：血隐蚤和印楝对恶性疟原虫3D7和DD2株具有协同作用。所述提取物组合使用比单独使用更可取。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊