Whole-transcriptome sequencing analysis to identify key non-coding RNAs in the pathogenesis of pulmonary tuberculosis.

IF 1.6 Q4 RESPIRATORY SYSTEM

Public Health Action Pub Date : 2026-08-24 eCollection Date: 2026-08-01 DOI:10.5588/pha.26.0020

M-B Liu, Y-L Guo, D-J Wu, Q-Q Ma, T Jiang, M Lin

{"title":"Whole-transcriptome sequencing analysis to identify key non-coding RNAs in the pathogenesis of pulmonary tuberculosis.","authors":"M-B Liu, Y-L Guo, D-J Wu, Q-Q Ma, T Jiang, M Lin","doi":"10.5588/pha.26.0020","DOIUrl":null,"url":null,"abstract":"Background: Non-coding RNAs (ncRNAs) have been shown to play essential roles in various human diseases, including pulmonary tuberculosis (TB). This study aims to investigate differentially expressed ncRNAs, explore their potential biological functions, and determine whether they can serve as biomarkers and therapeutic targets in TB.Methods: Whole-transcriptome RNA sequencing was performed on blood samples from TB patients and healthy controls (n = 3 per group). Differentially expressed mRNAs, long non-coding RNAs (lncRNAs), circular RNAs (circRNAs), and microRNAs (miRNAs) were identified, followed by Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses. A competing endogenous RNA (ceRNA) network was constructed, and hub genes were identified via protein-protein interaction network analysis using Cytoscape.Results: A total of 507 differentially expressed mRNAs, 107 differentially expressed lncRNAs, 253 differentially expressed circRNAs, and 38 differentially expressed miRNAs were identified. Functional enrichment revealed involvement in pathways related to cellular processes, mTOR signalling, and endocytosis. A circRNA-miRNA-mRNA ceRNA network was constructed, and hub mRNAs including RACK1, EIF3G, and RPS15 were identified.Conclusion: It is suggested that differentially expressed ncRNAs may play a role in the pathogenesis of TB. The circRNA (hsa_circ_0003340) and the hub mRNA (RACK1) are potential candidates for further exploration as biomarkers for TB infection. Moreover, the ceRNA network constructed in this study may provide new insights into the regulatory mechanisms of TB.","PeriodicalId":46239,"journal":{"name":"Public Health Action","volume":"16 3","pages":"281-287"},"PeriodicalIF":1.6000,"publicationDate":"2026-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13502504/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Public Health Action","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5588/pha.26.0020","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2026/8/1 0:00:00","PubModel":"eCollection","JCR":"Q4","JCRName":"RESPIRATORY SYSTEM","Score":null,"Total":0}

引用次数: 0

Abstract

Background: Non-coding RNAs (ncRNAs) have been shown to play essential roles in various human diseases, including pulmonary tuberculosis (TB). This study aims to investigate differentially expressed ncRNAs, explore their potential biological functions, and determine whether they can serve as biomarkers and therapeutic targets in TB.

Methods: Whole-transcriptome RNA sequencing was performed on blood samples from TB patients and healthy controls (n = 3 per group). Differentially expressed mRNAs, long non-coding RNAs (lncRNAs), circular RNAs (circRNAs), and microRNAs (miRNAs) were identified, followed by Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses. A competing endogenous RNA (ceRNA) network was constructed, and hub genes were identified via protein-protein interaction network analysis using Cytoscape.

Results: A total of 507 differentially expressed mRNAs, 107 differentially expressed lncRNAs, 253 differentially expressed circRNAs, and 38 differentially expressed miRNAs were identified. Functional enrichment revealed involvement in pathways related to cellular processes, mTOR signalling, and endocytosis. A circRNA-miRNA-mRNA ceRNA network was constructed, and hub mRNAs including RACK1, EIF3G, and RPS15 were identified.

Conclusion: It is suggested that differentially expressed ncRNAs may play a role in the pathogenesis of TB. The circRNA (hsa_circ_0003340) and the hub mRNA (RACK1) are potential candidates for further exploration as biomarkers for TB infection. Moreover, the ceRNA network constructed in this study may provide new insights into the regulatory mechanisms of TB.

查看原文本刊更多论文

全转录组测序分析鉴定肺结核发病机制中的关键非编码rna。

背景：非编码rna （ncRNAs）已被证明在包括肺结核（TB）在内的各种人类疾病中发挥重要作用。本研究旨在研究差异表达的ncrna，探索其潜在的生物学功能，并确定它们是否可以作为结核病的生物标志物和治疗靶点。方法：对TB患者和健康对照者的血液样本（每组n = 3）进行全转录组RNA测序。鉴定差异表达mrna、长链非编码rna （lncRNAs）、环状rna （circRNAs）和microRNAs (miRNAs)，随后进行基因本体和京都基因与基因组百科全书富集分析。构建了竞争性内源RNA （ceRNA）网络，并利用Cytoscape进行蛋白-蛋白相互作用网络分析，鉴定了枢纽基因。结果：共鉴定出507个差异表达mrna、107个差异表达lncrna、253个差异表达circrna和38个差异表达mirna。功能富集揭示了与细胞过程、mTOR信号传导和内吞作用相关的通路的参与。构建circRNA-miRNA-mRNA ceRNA网络，鉴定出RACK1、EIF3G和RPS15等枢纽mrna。结论：差异表达的ncRNAs可能在TB的发病机制中发挥作用。circRNA （hsa_circ_0003340）和hub mRNA （RACK1）是结核病感染生物标志物的潜在候选物，值得进一步探索。此外，本研究构建的ceRNA网络可能为结核病的调控机制提供新的见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Public Health Action RESPIRATORY SYSTEM-

自引率

0.00%

发文量

期刊介绍： Launched on 1 May 2011, Public Health Action (PHA) is an official publication of the International Union Against Tuberculosis and Lung Disease (The Union). It is an open access, online journal available world-wide to physicians, health workers, researchers, professors, students and decision-makers, including public health centres, medical, university and pharmaceutical libraries, hospitals, clinics, foundations and institutions. PHA is a peer-reviewed scholarly journal that actively encourages, communicates and reports new knowledge, dialogue and controversy in health systems and services for people in vulnerable and resource-limited communities — all topics that reflect the mission of The Union, Health solutions for the poor.