Micellar Enhanced Second Derivative Spectrofluorimetric Determination of Hydrochlorothiazide, Amlodipine, and Telmisartan in Fixed Dose Combination and Spiked Human Plasma

IF 2.8 4区化学 Q2 CHEMISTRY, ANALYTICAL

Luminescence Pub Date : 2026-08-23 DOI:10.1002/bio.70612

Suvarna Yenduri, Shashank H, Naga Prashant K

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引用次数: 0

Abstract

A very sensitive and eco-friendly second-derivative spectrofluorimetric method was developed for simultaneous analysis of hydrochlorothiazide (HCTZ), amlodipine besylate (AML), and telmisartan (TEL) in pharmaceutical products and human plasma. Native fluorescence of HCTZ (λex267/λem295 nm), AML (λex362/λem415 nm), and TEL (λex292/λem369 nm) was used together with fluorescence enhancement achieved through sodium lauryl sulfate micelles at optimum excitation/emission wavelengths for all other analytes being analyzed. Overlap of spectroscopic data was able to be resolved through second-derivative spectrofluorimetry without prior separation. Method validation was performed according to ICH Q2(R1) guidelines; results showed excellent linearity (R² > 0.999) across a concentration range of 3–18, 2–10, and 10–50 ng/mL for HCTZ, AML, and TEL, respectively. Accuracy, precision and robustness were demonstrated with %RSD values below two. Application of the method to pharmaceutical product and spiked plasma samples gave satisfactory recoveries with minimal matrix interference. Assessment of method greenness was conducted using AGREE Prep, MoGAPI, AGSA, SAMI, Ma Tool, CACI, and WECA metrics, all of which indicate superior environmental sustainability. The proposed method is simple, fast, low-cost, ultra-sensitive, and suitable for routine quality control and/or bioanalytical analysis.

查看原文本刊更多论文

胶束增强二阶导数荧光光谱法测定人血浆中氢氯噻嗪、氨氯地平和替米沙坦的固定剂量组合和加标。

建立了一种灵敏环保的二阶导数荧光光谱法，用于同时分析药品和人血浆中的氢氯噻嗪（HCTZ）、苯磺酸氨氯地平（AML）和替米沙坦（TEL）。使用HCTZ （λex267/λem295 nm）、AML （λex362/λem415 nm）和TEL （λex292/λem369 nm）的天然荧光，并通过十二烷基硫酸钠胶束在最佳激发/发射波长下对所有被分析物进行荧光增强。光谱数据的重叠可以通过二阶导数荧光光谱法解决，而无需事先分离。根据ICH Q2（R1）指南进行方法验证；结果表明，HCTZ、AML和TEL分别在3-18、2-10和10-50 ng/mL的浓度范围内呈良好的线性关系（R2 > 0.999）。准确度、精密度和稳健性均在%RSD值小于2时得到验证。该方法应用于药品和加标血浆样品，回收率令人满意，基质干扰最小。采用AGREE Prep、MoGAPI、AGSA、SAMI、Ma Tool、CACI和WECA指标对方法的绿色度进行了评估，所有这些指标都表明了优越的环境可持续性。该方法简单、快速、低成本、超灵敏，适用于常规质量控制和/或生物分析分析。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Luminescence 生物-生化与分子生物学

CiteScore

5.10

自引率

13.80%

发文量

248

审稿时长

3.5 months

期刊介绍： Luminescence provides a forum for the publication of original scientific papers, short communications, technical notes and reviews on fundamental and applied aspects of all forms of luminescence, including bioluminescence, chemiluminescence, electrochemiluminescence, sonoluminescence, triboluminescence, fluorescence, time-resolved fluorescence and phosphorescence. Luminescence publishes papers on assays and analytical methods, instrumentation, mechanistic and synthetic studies, basic biology and chemistry. Luminescence also publishes details of forthcoming meetings, information on new products, and book reviews. A special feature of the Journal is surveys of the recent literature on selected topics in luminescence.