Photodynamic Therapy for Toxoplasma gondii Infections Using Rose Bengal

IF 2.8 4区化学 Q2 CHEMISTRY, ANALYTICAL

Luminescence Pub Date : 2026-08-23 DOI:10.1002/bio.70583

Wen Li, Jiating Chen, Jie Li, Qingqing Zhang, Jihong Chen, Pengfei Zhang, Fufeng Liu

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引用次数: 0

Abstract

Toxoplasma gondii is a globally prevalent obligate intracellular protozoan parasite that causes severe zoonotic disease. Given the limitations of conventional drug therapies, including susceptibility to drug resistance, and significant toxic side effects, this study aimed to evaluate the efficacy of photodynamic therapy (PDT) combined with the classic photosensitizer Rose Bengal in eradicating T. gondii tachyzoites, whilst elucidating its underlying mechanism of action. Experimental results demonstrate that Rose Bengal efficiently induces the generation of both Type I and Type II reactive oxygen species (ROS) under light exposure, exhibiting potent phototoxic killing effects against T. gondii. Further transcriptomic analysis revealed that this mechanism involves Rose Bengal disrupting metabolic and motility-related signaling pathways in T. gondii, thereby programmatically inducing T. gondii cell death. Both in vivo and in vitro experiments confirmed that this approach effectively eradicates T. gondii while demonstrating favorable biosafety. This study provides experimental evidence for developing novel photodynamic therapies against T. gondii, expanding the application potential of photodynamic therapy in combating parasitic infections.

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玫瑰光动力治疗刚地弓形虫感染。

刚地弓形虫是一种全球流行的专性细胞内原生动物寄生虫，可引起严重的人畜共患疾病。鉴于传统药物治疗的局限性，包括对耐药性的敏感性和明显的毒副作用，本研究旨在评估光动力疗法（PDT）联合经典光敏剂玫瑰孟加拉（Rose Bengal）根除弓形虫速殖子的疗效，同时阐明其潜在的作用机制。实验结果表明，孟加拉玫瑰在光照下能有效诱导I型和II型活性氧（ROS）的产生，对弓形虫具有较强的光毒杀伤作用。进一步的转录组学分析表明，这一机制涉及到玫瑰花破坏弓形虫代谢和运动相关的信号通路，从而程序性地诱导弓形虫细胞死亡。体内和体外实验均证实该方法能有效根除弓形虫，同时具有良好的生物安全性。本研究为开发新型光动力治疗刚地弓形虫提供了实验依据，拓展了光动力治疗在抗寄生虫感染中的应用潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Luminescence 生物-生化与分子生物学

CiteScore

5.10

自引率

13.80%

发文量

248

审稿时长

3.5 months

期刊介绍： Luminescence provides a forum for the publication of original scientific papers, short communications, technical notes and reviews on fundamental and applied aspects of all forms of luminescence, including bioluminescence, chemiluminescence, electrochemiluminescence, sonoluminescence, triboluminescence, fluorescence, time-resolved fluorescence and phosphorescence. Luminescence publishes papers on assays and analytical methods, instrumentation, mechanistic and synthetic studies, basic biology and chemistry. Luminescence also publishes details of forthcoming meetings, information on new products, and book reviews. A special feature of the Journal is surveys of the recent literature on selected topics in luminescence.