Joseph Kreis, Jessica Wong, Yushan Yang, Palash Bharadwaj, Robert F Cooper, Mina Gaffney, Nivedhitha Govindasamy, Amy L Green, Brian P Higgins, Yu You Jiang, Angelos Kalitzeos, Michel Michaelides, Jessica I W Morgan, Jason Porter, Jeremy D Rogers, Ian Rosenthal, Ramkumar Sabesan, Kaitlyn A Sapoznik, Alexander W Schill, Ryan D Sochol, Yiyi Wang, Raymond L Warner, Emma Warr, Benjamin J Wendel, Niamh Wynne, Peiluo Xu, Sergey Tarima, Daniel X Hammer, Anant Agrawal, Jacque L Duncan, Joseph Carroll
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引用次数: 0
Abstract
Purpose: To describe the use of a model eye-mounted retinal phantom to support adaptive optics scanning light ophthalmoscope (AOSLO) imaging in a multicenter clinical trial (NCT05537220).
Methods: Six three-dimensional-printed retinal phantoms were mounted in model eyes. These phantoms contain equally spaced arrays of reflective "cone-like" structures mimicking foveal cone photoreceptor spacing. Intrasession repeatability of phantom imaging was assessed for all six phantoms on two custom AOSLOs. One phantom was also imaged on two additional AOSLOs to assess interdevice reproducibility. Lastly, each of the six phantoms was imaged longitudinally at their respective trial sites.
Results: Intrasession repeatability of phantom-derived horizontal "cone" spacing measures was high for two different AOSLOs. However, intersystem differences were found in the average horizontal "cone" spacing of five of eight patches assessed. Across four unique AOSLOs, intersystem differences were found in the average horizontal "cone" spacing of seven of eight patches assessed. A linear regression analysis of longitudinal phantom imaging with a single AOSLO revealed few significant changes in horizontal "cone" spacing over the course of about 2 years. Similar results were observed for vertical "cone" spacing measures across the three experiments.
Conclusions: Longitudinal spacing measurements were generally stable in our study. However, small but significant differences in spacing were observed between systems. The approach described here may facilitate combining cone metrics extracted from images acquired using different AOSLOs in multicenter studies.
Translational relevance: Cross-system validation strategies can support the use of AOSLO imaging in multicenter clinical trials.
期刊介绍：
Translational Vision Science & Technology (TVST), an official journal of the Association for Research in Vision and Ophthalmology (ARVO), an international organization whose purpose is to advance research worldwide into understanding the visual system and preventing, treating and curing its disorders, is an online, open access, peer-reviewed journal emphasizing multidisciplinary research that bridges the gap between basic research and clinical care. A highly qualified and diverse group of Associate Editors and Editorial Board Members is led by Editor-in-Chief Marco Zarbin, MD, PhD, FARVO.
The journal covers a broad spectrum of work, including but not limited to:
Applications of stem cell technology for regenerative medicine,
Development of new animal models of human diseases,
Tissue bioengineering,
Chemical engineering to improve virus-based gene delivery,
Nanotechnology for drug delivery,
Design and synthesis of artificial extracellular matrices,
Development of a true microsurgical operating environment,
Refining data analysis algorithms to improve in vivo imaging technology,
Results of Phase 1 clinical trials,
Reverse translational ("bedside to bench") research.
TVST seeks manuscripts from scientists and clinicians with diverse backgrounds ranging from basic chemistry to ophthalmic surgery that will advance or change the way we understand and/or treat vision-threatening diseases. TVST encourages the use of color, multimedia, hyperlinks, program code and other digital enhancements.
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