Physicochemical Analysis of Extracellular Nanovesicles and Exosome-Like Nanovesicles

IF 0.4 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry Pub Date : 2026-08-25 DOI:10.1134/S1990750826600287

K. S. Evmenov, E. A. Tkachenko, S. A. Brezgin, A. P. Kostyusheva, N. I. Ponomareva, P. A. Demina, L. Fatkhutdinova, M. V. Zyuzin, D. S. Kostyushev

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引用次数: 0

Abstract

Extracellular vesicles (EVs) represent a promising tool for diagnostics and therapy, but high heterogeneity limits their practical use. In this work, a comparative study of the physicochemical properties of native EVs secreted by HEK-293T cells and exosome-like nanovesicles (EMNVs) obtained by extrusion of whole HEK-293T cells was carried out. Using transmission electron microscopy, dynamic and electrophoretic light scattering, and surface-enhanced Raman spectroscopy, the authors found that EMNVs form a more homogeneous population with an average diameter of ~115 nm and a high negative charge (−22.9 mV), whereas native EVs are more heterogeneous in size and have a lower zeta potential (−12.1 mV). Spectral analysis revealed the presence of nucleic acid signals in native EVs and their absence in EMNVs, indicating differences in molecular composition due to different biogenesis mechanisms. The obtained data demonstrate that EMNVs are a more standardized and colloidally stable alternative to native EVs, making them a promising platform for the development of drug-delivery systems.

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细胞外纳米囊泡和外泌体样纳米囊泡的理化分析

细胞外囊泡（EVs）是一种很有前途的诊断和治疗工具，但高度的异质性限制了它们的实际应用。本研究比较了HEK-293T细胞分泌的天然ev和通过挤压整个HEK-293T细胞获得的外泌体样纳米囊泡（EMNVs）的理化性质。通过透射电子显微镜、动态和电泳光散射以及表面增强拉曼光谱，作者发现emnv形成了一个更均匀的群体，平均直径为~115 nm，负电荷高（- 22.9 mV），而天然ev在尺寸上更不均匀，具有更低的zeta电位（- 12.1 mV）。光谱分析显示，天然ev中存在核酸信号，而emnv中不存在核酸信号，表明不同生物发生机制导致分子组成的差异。获得的数据表明，与天然电动汽车相比，emnv是一种更标准化、胶体更稳定的替代品，使其成为开发药物输送系统的有前途的平台。

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来源期刊

Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry BIOCHEMISTRY & MOLECULAR BIOLOGY-

CiteScore

1.10

自引率

0.00%

发文量

期刊介绍： Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry covers all major aspects of biomedical chemistry and related areas, including proteomics and molecular biology of (patho)physiological processes, biochemistry, neurochemistry, immunochemistry and clinical chemistry, bioinformatics, gene therapy, drug design and delivery, biochemical pharmacology, introduction and advertisement of new (biochemical) methods into experimental and clinical medicine. The journal also publishes review articles. All issues of the journal usually contain solicited reviews.