Comparative antioxidant efficacy of D-Cateslytin combined with modified triple antibiotic paste and calcium hydroxide: an in vitro study.

IF 2.7 Q3 DENTISTRY, ORAL SURGERY & MEDICINE

Frontiers in dental medicine Pub Date : 2026-08-05 eCollection Date: 2026-01-01 DOI:10.3389/fdmed.2026.1921120

Alen Romel, Harish Shetty, Vidhyadhara Shetty, Shravan Kini, Nikhil Harikrishnan, Ashwini Prabhu

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Abstract

Background: Successful endodontic treatment depends on effective elimination of microorganisms and the creation of a favorable biological environment for periapical healing. Conventional intracanal medicaments such as calcium hydroxide and Modified Triple Antibiotic Paste (MTAP) possess antimicrobial activity; however, their ability to modulate oxidative stress within the root canal environment is limited. D-Cateslytin, a peptide derived from Chromogranin A, has shown promising antimicrobial activity against various oral pathogens and may also contribute to antioxidant effects when used in combination with traditional intracanal medicaments.

Aim: To evaluate and compare the antioxidant activity of D-Cateslytin in combination with calcium hydroxide and MTAP.

Materials and methods: An in vitro experimental study was conducted using six experimental groups: control, D-Cateslytin, calcium hydroxide, MTAP, D-Cateslytin + calcium hydroxide, and D-Cateslytin + MTAP. The antioxidant activity was assessed by performing 2,2-diphenyl-1-picrylhydrazyl(DPPH) free radical scavenging activity and nitric oxide (NO) scavenging activity. The absorbance was measured at 517 nm for DPPH and 540 nm for NO.

Statistical analysis: Data were analysed using one-way ANOVA followed by Bonferroni post hoc test (p < 0.05).

Results: Both D-Cateslytin combination groups demonstrated greater antioxidant activity than calcium hydroxide and MTAP alone. Although the D-Cateslytin + MTAP group exhibited the highest antioxidant activity, it was not significantly different from D-Cateslytin alone or D-Cateslytin + calcium hydroxide.

Conclusion: D-Cateslytin exhibited superior antioxidant activity compared to conventional intracanal medicaments. The addition of D-Cateslytin to calcium hydroxide and MTAP may enhance the biological properties of intracanal medicaments and could contribute to improved endodontic treatment outcomes.

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D-Cateslytin与改性三联抗生素糊剂和氢氧化钙联合抗氧化效果的体外比较研究。

背景：成功的根管治疗依赖于微生物的有效清除和根尖周愈合良好的生物环境的创造。传统的鼻腔内药物如氢氧化钙和改性三联抗生素膏（MTAP）具有抗菌活性；然而，它们调节根管环境中氧化应激的能力是有限的。D-Cateslytin是一种从嗜铬粒蛋白a中提取的肽，对多种口腔病原体具有良好的抗菌活性，当与传统的肛管内药物联合使用时，也可能有助于抗氧化作用。目的：评价和比较D-Cateslytin与氢氧化钙、MTAP复合的抗氧化活性。材料与方法：采用对照、D-Cateslytin、氢氧化钙、MTAP、D-Cateslytin +氢氧化钙、D-Cateslytin + MTAP 6个实验组进行体外实验研究。通过对2,2-二苯基-1-苦味酰肼（DPPH）自由基的清除和对一氧化氮（NO）的清除来评估其抗氧化活性。DPPH的吸光度为517 nm， NO的吸光度为540 nm。统计分析：采用单因素方差分析和Bonferroni事后检验(p)对数据进行分析。结果：D-Cateslytin联合用药组的抗氧化活性均高于氢氧化钙和MTAP单独用药组。虽然D-Cateslytin + MTAP组的抗氧化活性最高，但与D-Cateslytin单独或D-Cateslytin +氢氧化钙没有显著差异。结论：D-Cateslytin具有较好的抗氧化活性。将D-Cateslytin添加到氢氧化钙和MTAP中可以提高管内药物的生物学特性，并有助于改善根管治疗效果。

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