[Drug resistance of clinical Mycobacterium tuberculosis isolates and molecular characteristics of rifampicin-resistant isolates from elderly pulmonary tuberculosis patients in Beijing, 2024].
Y Deng, H H Miao, M D Pang, J Zhang, X Y Yang, S S Chen, J L Yi, Y X Ren, R F Fan, X X He, C Y Li, J Li
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引用次数: 0
Abstract
Objective: To analyze the drug resistance spectrum of clinical isolates of Mycobacterium (M.) tuberculosis and the mutations in drug resistance-related genes in the genomic sequences of rifampicin-resistant isolates from elderly pulmonary tuberculosis (TB) patients in Beijing in 2024. Methods: A total of 474 M. tuberculosis isolates from elderly pulmonary TB patients were included. Drug susceptibility testing against 10 anti-TB drugs was performed with microbroth dilution method. Whole-genome sequencing (WGS) was performed for 27 rifampicin-resistant isolates to systematically characterize resistance-associated gene mutations and genotype-phenotype consistency. Results: The total drug resistance rate was 24.26% in the 474 M. tuberculosis isolates. The resistance rate was significantly higher in retreatment patients (44.00%) than in new patients (23.16%), and a higher multidrug resistance rate was observed in men (6.75% vs. 2.03%). WGS revealed that all the rifampicin-resistant strains belonged to lineage 2 (East Asian lineage), with rpoB p.Ser450Leu (55.56%) being the most prevalent mutation. Consistency between WGS and phenotypic drug susceptibility was 96.30% for isoniazid, pyrazinamide, and rifampicin, but was low for ethambutol (22.22%). Conclusions: The drug resistance of M. tuberculosis was severe in elderly pulmonary TB patients in Beijing, which was associated with treatment history and gender. Rifampicin-resistant strains showed lineage 2 clustering, indicating a risk for local transmission. The resistance genotypes detected by WGS were highly consistent with the phenotypic drug susceptibility of isoniazid and pyrazinamide, but showed low consistency with ethambutol.
期刊介绍：
Chinese Journal of Epidemiology, established in 1981, is an advanced academic periodical in epidemiology and related disciplines in China, which, according to the principle of integrating theory with practice, mainly reports the major progress in epidemiological research. The columns of the journal include commentary, expert forum, original article, field investigation, disease surveillance, laboratory research, clinical epidemiology, basic theory or method and review, etc.
The journal is included by more than ten major biomedical databases and index systems worldwide, such as been indexed in Scopus, PubMed/MEDLINE, PubMed Central (PMC), Europe PubMed Central, Embase, Chemical Abstract, Chinese Science and Technology Paper and Citation Database (CSTPCD), Chinese core journal essentials overview, Chinese Science Citation Database (CSCD) core database, Chinese Biological Medical Disc (CBMdisc), and Chinese Medical Citation Index (CMCI), etc. It is one of the core academic journals and carefully selected core journals in preventive and basic medicine in China.
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