PASTA: versatile tyramide-oligonucleotide amplification for multimodal spatial biology

IF 28.3 1区生物学 Q1 BIOCHEMICAL RESEARCH METHODS

Nature Methods Pub Date : 2026-08-17 DOI:10.1038/s41592-026-03200-z

Hendrik A. Michel, Paige McCallum, Wenrui Wu, Huaying Qiu, Jia Le Lee, Shuli Luo, Chi Ngai Chan, Johanna Schaffenrath, Yao Yu Yeo, Stephanie Pei Tung Yiu, Yang Wang, Lindsay Parmelee, Hongbo Wang, Tania Pannellini, Melinda Burgess, Nourhan El Ahmar, Zoe Xiaozhu Zhang, Colm Keane, Tony Kiat Hon Lim, Sabina Signoretti, Sonia Victoria Del Rincon, Bo Zhao, David R. McIlwain, Yunhao Bai, Fei Chen, Roberto Chiarle, Sizun Jiang

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引用次数: 0

Abstract

Spatial proteomics is limited by detection sensitivity, multiplexing and multimodal integration, leaving a gap between discovery and clinical assays. Here we present protein and nucleic acid serial tyramide amplification (PASTA), using horseradish peroxidase-mediated oligonucleotide deposition and cyclical imaging for high-plex, multimodal spatial profiling. Compatible with conjugated antibodies and in situ hybridization, PASTA enables simultaneous protein and RNA codetection from formalin-fixed, paraffin-embedded samples, providing a cost-effective bridge from discovery to clinical validation. PASTA is a platform-agnostic signal amplification approach for multiplexed imaging across modalities.

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面食：多模态空间生物学的多用途酪酰胺寡核苷酸扩增。

空间蛋白质组学受到检测灵敏度、多路复用和多模式集成的限制，在发现和临床分析之间留下了差距。在这里，我们提出了蛋白质和核酸序列酰胺扩增（PASTA），使用辣根过氧化物酶介导的寡核苷酸沉积和循环成像进行高plex，多模态空间分析。PASTA与偶联抗体和原位杂交兼容，可以同时对福尔马林固定的石蜡包埋样品进行蛋白质和RNA的共检测，为从发现到临床验证提供了一个具有成本效益的桥梁。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Nature Methods 生物-生化研究方法

CiteScore

58.70

自引率

1.70%

发文量

326

审稿时长

1 months

期刊介绍： Nature Methods is a monthly journal that focuses on publishing innovative methods and substantial enhancements to fundamental life sciences research techniques. Geared towards a diverse, interdisciplinary readership of researchers in academia and industry engaged in laboratory work, the journal offers new tools for research and emphasizes the immediate practical significance of the featured work. It publishes primary research papers and reviews recent technical and methodological advancements, with a particular interest in primary methods papers relevant to the biological and biomedical sciences. This includes methods rooted in chemistry with practical applications for studying biological problems.